Germline DLST Variants Promote Epigenetic Modifications in Pheochromocytoma-Paraganglioma.

Abstract

CONTEXT:

Pheochromocytomas and paragangliomas (PPGLs) are neuroendocrine tumors in which altered central metabolism appears to be a major driver of tumorigenesis, and many PPGL genes encode proteins involved in the tricarboxylic acid (TCA) cycle.

OBJECTIVE/DESIGN:

While about 40% of PPGL cases carry a variant in a known gene, many cases remain unexplained. In patients with unexplained PPGL showing clear evidence of a familial burden or multiple tumors, we aimed to identify causative factors using genetic analysis of patient DNA and functional analyses of identified DNA variants in patient tumor material and engineered cell lines.

PATIENTS AND SETTING:

Patients with a likely familial cancer burden of pheochromocytomas and/or paragangliomas and under investigation in a clinical genetic and clinical research setting in university hospitals.

RESULTS:

While investigating unexplained PPGL cases, we identified a novel variant, c.1151C>T, p.(Pro384Leu), in exon 14 of the gene encoding dihydrolipoamide S-succinyltransferase (DLST), a component of the multi-enzyme complex 2-oxoglutarate dehydrogenase. Targeted sequence analysis of further unexplained cases identified a patient carrying a tumor with compound heterozygous variants in DLST, consisting of a germline variant, c.1121G>A, p.(Gly374Glu), together with a somatic missense variant identified in tumor DNA, c.1147A>G, p.(Thr383Ala), both located in exon 14. Using a range of in silico and functional assays we show that these variants are predicted to be pathogenic, profoundly impact enzyme activity, and result in DNA hypermethylation.

CONCLUSIONS:

The identification and functional analysis of these DLST variants further validates DLST as an additional PPGL gene involved in the TCA cycle.

• Authors: Alexandre Buffet, Juan Zhang, Heggert Rebel, Eleonora P M Corssmit, Jeroen C Jansen, Erik F Hensen, Judith V M G Bovée, Aurélien Morini, Anne-Paule Gimenez-Roqueplo, Frederik J Hes, Peter Devilee, Judith Favier, Jean-Pierre Bayley
• Journal: The Journal of clinical endocrinology and metabolism (J Clin Endocrinol Metab) Vol. 106 Issue 2 Pg. 459-471 (01 23 2021) ISSN: 1945-7197 [Electronic] United States
• PMID: 33180916 (Publication Type: Case Reports, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © The Author(s) 2020. Published by Oxford University Press on behalf of the Endocrine Society. All rights reserved. For permissions, please e-mail: [email protected].

Chemical References

• Biomarkers
• Acyltransferases
• dihydrolipoamide succinyltransferase

Topics

• Acyltransferases (genetics)
• Adrenal Gland Neoplasms (genetics, pathology)
• Adult
• Aged
• Biomarkers (analysis)
• DNA Methylation
• Epigenesis, Genetic
• Female
• Genetic Predisposition to Disease
• Germ-Line Mutation
• Humans
• Male
• Paraganglioma (genetics, pathology)
• Pheochromocytoma (genetics, pathology)
• Prognosis
• Young Adult