Abstract | AIMS: METHODS: FINDINGS: The results showed that the expression of circ-HECTD1 and tumor necrosis factor receptor-associated factor 3 ( TRAF3) was remarkably up-regulated, while miR-133b was down-regulated in oxygen- glucose deprivation (OGD)-induced HT22 cells and mouse middle cerebral artery occlusion (MCAO) model. circ-HECTD1 knockdown relieved OGD-caused neuronal cell death in vitro. Simultaneously, circ-HECTD1 knockdown improved cerebral infarction volume and neuronal apoptosis in MCAO mice. circ-HECTD1 was able to negatively regulate the expression of miR-133b, and TRAF3 is one of the targets of miR-133b. Upregulation of miR-133b inhibited the expression of TRAF3 in OGD-stimulated cells, whereas circ-HECTD1 upregulation reversed this effect. Furthermore, upregulation of miR-133 was able to inhibit OGD-caused cell apoptosis and NF-κB activation, whereas upregulation of circ-HECTD1 attenuated these effects of miR-133b mimics. SIGNIFICANCE: Taken together, circ-HECTD1 knockdown inhibited the expression of TRAF3 by targeting miR-133b, thereby attenuating neuronal injury caused by cerebral ischemia.
|
Authors | Quande Dai, Yu Ma, Zhonghai Xu, Liang Zhang, Hong Yang, Qin Liu, Jingzhong Wang |
Journal | Life sciences
(Life Sci)
Vol. 264
Pg. 118626
(Jan 01 2021)
ISSN: 1879-0631 [Electronic] Netherlands |
PMID | 33148417
(Publication Type: Journal Article)
|
Copyright | Copyright © 2020 Elsevier Inc. All rights reserved. |
Chemical References |
- Blood Glucose
- MicroRNAs
- Mirn133 microRNA, mouse
- RNA, Circular
- TNF Receptor-Associated Factor 3
- Hectd1 protein, mouse
- Ubiquitin-Protein Ligases
- Casp3 protein, mouse
- Caspase 3
- Oxygen
|
Topics |
- Animals
- Apoptosis
- Blood Glucose
(metabolism)
- Brain
(pathology)
- Brain Ischemia
(pathology)
- Caspase 3
(metabolism)
- Down-Regulation
- Gene Knockdown Techniques
- Ischemic Stroke
(genetics)
- Male
- Mice
- Mice, Inbred C57BL
- MicroRNAs
(genetics)
- Middle Cerebral Artery
(pathology)
- Neurons
(pathology)
- Neuroprotection
- Oxygen
(metabolism)
- RNA, Circular
(genetics)
- Reperfusion Injury
(pathology)
- TNF Receptor-Associated Factor 3
(genetics)
- Ubiquitin-Protein Ligases
(genetics)
|