A general strategy towards personalized nanovaccines based on fluoropolymers for post-surgical cancer immunotherapy.

Abstract

Cancer metastases and recurrence after surgical resection remain an important cause of treatment failure. Here we demonstrate a general strategy to fabricate personalized nanovaccines based on a cationic fluoropolymer for post-surgical cancer immunotherapy. Nanoparticles formed by mixing the fluoropolymer with a model antigen ovalbumin, induce dendritic cell maturation via the Toll-like receptor 4 (TLR4)-mediated signalling pathway, and promote antigen transportation into the cytosol of dendritic cells, which leads to an effective antigen cross-presentation. Such a nanovaccine inhibits established ovalbumin-expressing B16-OVA melanoma. More importantly, a mix of the fluoropolymer with cell membranes from resected autologous primary tumours synergizes with checkpoint blockade therapy to inhibit post-surgical tumour recurrence and metastases in two subcutaneous tumour models and an orthotopic breast cancer tumour. Furthermore, in the orthotopic tumour model, we observed a strong immune memory against tumour rechallenge. Our work offers a simple and general strategy for the preparation of personalized cancer vaccines to prevent post-operative cancer recurrence and metastasis.

• Authors: Jun Xu, Jia Lv, Qi Zhuang, Zongjin Yang, Zhiqin Cao, Ligeng Xu, Pei Pei, Chenya Wang, Hanfei Wu, Ziliang Dong, Yu Chao, Chao Wang, Kai Yang, Rui Peng, Yiyun Cheng, Zhuang Liu
• Journal: Nature nanotechnology (Nat Nanotechnol) Vol. 15 Issue 12 Pg. 1043-1052 (12 2020) ISSN: 1748-3395 [Electronic] England
• PMID: 33139933 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cancer Vaccines
• Fluorocarbon Polymers
• Polyethyleneimine
• Ovalbumin

Topics

• Animals
• Cancer Vaccines (chemistry, therapeutic use)
• Cells, Cultured
• Female
• Fluorocarbon Polymers (chemistry, therapeutic use)
• Immunotherapy
• Melanoma, Experimental (immunology, prevention & control, surgery)
• Mice, Inbred BALB C
• Mice, Inbred C57BL
• Nanoparticles (therapeutic use)
• Ovalbumin (chemistry, therapeutic use)
• Polyethyleneimine (chemistry, therapeutic use)