Kidney stones, also known as
calcium oxalate (CaOx)
nephrolithiasis, are often asymptomatic, leading to kidney injury and
renal failure complications.
Corilagin is a
gallotannin found in various plants and is known to elicit various biological activities. The present study aimed to elucidate the renoprotective effect of
corilagin against the rats' renal stones deposition. The rats were induced for
nephrolithiasis (CaOx deposition) using 0.75%
ethylene glycol in their
drinking water. Then, they were treated with
corilagin at 50 and 100 mg/kg/day for 4 weeks. At the end of the experimental period, the rats were killed; blood and renal tissues were collected for various histological, biochemical, and gene expression analyses. The results demonstrated that the rats had
renal calculi displaying a significant increase in serum
creatinine (59.39 μmol/L) and blood
urea nitrogen (19.03 mmol/L) levels compared with controls. Moreover, the
malondialdehyde (13.29 nmol/mg) level was found to increase with a profound reduction in
antioxidants' activities with upregulated inflammatory
cytokines. In contrast, the RT-PCR and immunohistochemistry analysis demonstrated a substantial reduction in cell survival markers
PPAR-γ and PI3K/Akt with an apparent increase in apoptosis markers genes expressions in rats suffering from renal stones. Thus, the present study results suggest that
corilagin could suppress renal CaOx crystal-induced oxidative stress, inflammatory response, and apoptosis via
PPAR-γ and PI3K/Akt-mediated pathway.