Dermal mesenchymal stem cells promoted adhesion and migration of endothelial cells by integrin in psoriasis.

Abstract

The unusual dilatation of dermal capillaries and angiogenesis played important roles in psoriasis. Some genes and proteins of dermal mesenchymal stem cells (DMSCs) from psoriasis are abnormal and related to the function of endothelial cells (ECs). The present study was aimed to evaluate whether psoriatic DMSCs could affect adhesion and migration of ECs through neovascularization-related integrins in psoriasis. Human DMSCs, collected from psoriasis lesions and healthy skin, respectively, were cocultured with human umbilical vein endothelial cells (HUVECs). The expression levels of three integrins, that is, αvβ3, αvβ5, and α5β1 in HUVECs were tested by quantitative real-time polymerase chain reaction and Western blot analysis. The adhesion and migration of HUVECs were detected by adhesion assay and migration assay. The results showed that in psoriasis group, the expression of αVβ3 and α5β1 of HUVECs markedly increased 2.50- and 3.71-fold in messenger RNA levels, and significantly increased 1.63- and 1.92-fold in protein levels, comparing to healthy control group (all p < .05). But β5 was not significantly different between the two groups (p > .05). In addition, compared with control, psoriatic DMSCs promoted HUVECs adhesion by 1.62-fold and migration by 2.91-fold (all p < .05). In conclusion, psoriatic DMSCs impact HUVECs adhesion and migration by upregulating the expression of integrins αVβ3 and α5β1.

• Authors: Qixin Han, Xuping Niu, Ruixia Hou, Junqin Li, Yamin Liu, Xiaofang Li, Juan Li, Yan Li, Kaiming Zhang, Yan Wu
• Journal: Cell biology international (Cell Biol Int) Vol. 45 Issue 2 Pg. 358-367 (Feb 2021) ISSN: 1095-8355 [Electronic] England
• PMID: 33079476 (Publication Type: Journal Article)
• Copyright: © 2020 International Federation for Cell Biology.

Chemical References

• ITGA5 protein, human
• Integrins

Topics

• Adolescent
• Adult
• Cell Adhesion
• Child
• Female
• Human Umbilical Vein Endothelial Cells
• Humans
• Infant
• Integrins (physiology)
• Male
• Mesenchymal Stem Cells
• Neovascularization, Pathologic
• Psoriasis (metabolism, pathology)
• Skin (metabolism, pathology)
• Young Adult