Abstract | BACKGROUND: AIM OF THE STUDY: The aim of this study is to determine whether ANXA1 is regulated by ADT and participates in PCa progression after ADT, and to explore the possible mechanism of ANXA1-mediated PCa migration. METHODS: Expression of ANXA1 and androgen receptor (AR) in PCa cell lines and tissues was detected, and the association between these two proteins were analyzed. Expression of ANXA1 was evaluated after AR knockdown or AR inhibition in PCa cell lines. Cell migration of PCa cell liness after ANXA1 knockdown or overexpression was determined by in vitro migration assay. Transcriptome analysis was used to explore the possible mechanism of ANXA1-mediated PCa migration. RESULTS: ANXA1 expression in PCa cell lines and tissues was reversely associated with AR. In vitro studies revealed an increase in ANXA1 expression after AR knockdown or treatment with AR antagonist. Moreover, functional assays indicated that ANXA1 knockdown in PCa cells significantly inhibited cell migration, while ANXA1 overexpression in PCa cells significantly accelerated cell migration. Transcriptome analysis showed that ANXA1 regulated multiple genes involved in cell junction organization, such as CADM1, LIMCH1 and PPM1F. CONCLUSIONS: Our results indicate that ADT might accelerate PCa metastasis via ANXA1 expression and PCa cell migration.
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Authors | Wenjie Yang, Ke Wang, Jianbin Ma, Ke Hui, Wei Lv, Zhenkun Ma, Mengxi Huan, Lin Luo, Xinyang Wang, Lei Li, Yule Chen |
Journal | Archives of medical research
(Arch Med Res)
Vol. 52
Issue 2
Pg. 174-181
(02 2021)
ISSN: 1873-5487 [Electronic] United States |
PMID | 33059953
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2021 IMSS. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- ANXA1 protein, human
- AR protein, human
- Androgen Antagonists
- Annexin A1
- Benzamides
- Nitriles
- RNA, Messenger
- Receptors, Androgen
- Phenylthiohydantoin
- enzalutamide
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Topics |
- Androgen Antagonists
(pharmacology, therapeutic use)
- Annexin A1
(biosynthesis, genetics, metabolism)
- Benzamides
- Cell Line, Tumor
- Cell Movement
(drug effects)
- Humans
- Male
- Neoplasm Metastasis
- Nitriles
- PC-3 Cells
- Phenylthiohydantoin
(analogs & derivatives, pharmacology)
- Prostatic Neoplasms
(drug therapy, genetics, metabolism, pathology)
- RNA, Messenger
(genetics, metabolism)
- Receptors, Androgen
(biosynthesis, genetics, metabolism)
- Signal Transduction
- Up-Regulation
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