ALDH-1-positive cells exhibited a radioresistant phenotype that was enhanced with hypoxia in cervical cancer.

Abstract

BACKGROUND:

We have previously found there was a small subpopulation of cells with cancer stem cell-like phenotype ALDH-1 in cervical cancer. Radiotherapy has been applied in most of the cervical cancer. However,the mechanisms underlying radioresistance still remained elusive. Our study is to explore whether ALDH+ cell promotes radioresistance by hypoxia.

METHODS:

Cells were respectively cultured in hypoxia and normoxia environment and analyzed for marker stability, and cell cycle distribution.

RESULTS:

Cell growth, apoptosis, cell cycle, sphere formation were affected by hypoxia. ALDH-1 and CHK2 were upregulated after hypoxia.

CONCLUSIONS:

Here we show that ALDH-1 positive cells contribute to cervical carcinoma radioresistance through preferential activation of the DNA damage checkpoint response and an increase in DNA repair capacity. The fraction of these cells is enriched after radiation in cervical carcinoma.

• Authors: Tingting Yao, Xueling Weng, Yao Yao, Chunxian Huang, Jing Li, Yongpai Peng, Rongchun Lin, Zhongqiu Lin
• Journal: BMC cancer (BMC Cancer) Vol. 20 Issue 1 Pg. 891 (Sep 17 2020) ISSN: 1471-2407 [Electronic] England
• PMID: 32942996 (Publication Type: Journal Article)

Chemical References

• Aldehyde Dehydrogenase 1 Family

Topics

• Aldehyde Dehydrogenase 1 Family (metabolism)
• Animals
• Cell Hypoxia
• Cell Proliferation
• Disease Models, Animal
• Female
• Humans
• Mice
• Mice, Nude
• Phenotype
• Uterine Cervical Neoplasms (genetics)