The Expression Profile of mRNA and tRNA Genes in Splenocytes and Neutrophils after In Vivo Delivery of Antitumor Short Hairpin RNA of Indoleamine 2,3- Dioxygenase.

Abstract

RNA-based therapeutics are considered as novel treatments for human diseases. Our previous study demonstrated that treatment with short-hairpin RNA against Ido1 (IDO shRNA) suppresses tumor growth, detects Th1-bias immune responses, and elevates expression of tryptophan transfer RNA (tRNATrp) in total splenocytes. In addition, depletion of Ly6g+ neutrophils attenuates the effect of IDO shRNA. The aim of this study was to investigate the regulatory network and the expression profile of tRNAs and other non-coding RNAs in IDO shRNA-treated spleens. The total splenocytes and magnetic bead-enriched splenic neutrophils were collected from the lung tumor bearing mice, which were treated with IDO shRNA or scramble IDO shRNA, and the collected cells were subsequently subjected to RNA sequencing. The gene ontology analysis revealed the different enrichment pathways in total splenocytes and splenic neutrophils. Furthermore, the expression of tRNA genes was identified and validated. Six isoacceptors of tRNA, with different expression patterns between total splenocytes and splenic neutrophils, were observed. In summary, our findings not only revealed novel biological processes in IDO shRNA-treated total splenocytes and splenic neutrophils, but the identified tRNAs and other non-coding RNAs may contribute to developing a novel biomarker gene set for evaluating the clinical efficiency of RNA-based cancer immunotherapies.

• Authors: Ming-Shyan Huang, Ya-Ling Hsu, I-Jeng Yeh, Kuan-Ting Liu, Meng-Chi Yen
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 21 Issue 18 (Sep 13 2020) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 32933162 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• Biomarkers, Tumor
• Indoleamine-Pyrrole 2,3,-Dioxygenase
• RNA, Messenger
• RNA, Small Interfering
• RNA, Transfer

Topics

• Animals
• Antineoplastic Agents (administration & dosage)
• Biomarkers, Tumor (genetics)
• Cell Line, Tumor
• Female
• Gene Expression Regulation (drug effects, genetics)
• Gene Ontology
• Indoleamine-Pyrrole 2,3,-Dioxygenase (administration & dosage, genetics)
• Lung Neoplasms (genetics)
• Mice
• Mice, Inbred C57BL
• Neutrophils (drug effects, physiology)
• RNA, Messenger (genetics)
• RNA, Small Interfering (administration & dosage, genetics)
• RNA, Transfer (genetics)
• Spleen (drug effects, physiology)