Abstract | Purpose: The retina is a commonly used model for angiogenesis research due to its special characteristics. Oxygen-induced retinopathy (OIR) provides a useful model to study ischemia-induced neovascularization (NV) and to develop anti-angiogenic therapeutics. The purpose of this study was to develop a simple, accurate, and less-subjective quantification method for retinal NV in the OIR model. Methods: To address this challenge, we combined the conventional vascular staining and BrdU labeling of newly formed vascular cells to detect and analyze retinal NV. With daily injections of BrdU, which was incorporated into the DNA of newly formed retinal vessels under the OIR condition, ischemia-induced retinal neovasculature with BrdU labeling was distinguished from pre-existing vasculature and accurately quantified using the ImageJ program. Results: Conclusions: Translational Relevance: Quantitative, straightforward, and objective observation and evaluation of pathologic neovasculature are important to study the pathogenesis of NV and therapeutic effects using animal models.
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Authors | Yan Shao, Jianglei Chen, Xiao-Rong Li, Jian-Xing Ma |
Journal | Translational vision science & technology
(Transl Vis Sci Technol)
Vol. 9
Issue 9
Pg. 4
(08 2020)
ISSN: 2164-2591 [Print] United States |
PMID | 32879761
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2020 The Authors. |
Chemical References |
- Vascular Endothelial Growth Factor A
- Bromodeoxyuridine
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Topics |
- Animals
- Bromodeoxyuridine
- Disease Models, Animal
- Mice
- Retinal Neovascularization
- Retinal Vessels
- Vascular Endothelial Growth Factor A
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