Abstract |
Our previous study has recorded that the recombinant protein of Nile tilapia (Oreochromis niloticus) galectin-2 (rOnGal-2) can enhance immune response against Streptococcus agalactiae (S.agalactiae) infection in vitro. In this study, we further explored the effects of OnGal-2 in immune response against bacterial infection in vivo. The administration of rOnGal-2 could improve serum antibacterial activity (ALKP, ACP, and LZM) and antioxidant capacity (CAT, POD, and SOD). After S. agalactiae infection, rOnGal-2 injection could reduce bacterial burden and decrease tissue damage in head kidney, spleen, and liver of tilapia. Also, rOnGal-2 regulated the inflammatory-related genes expression including IL-6, IL-8 and IL-10 during bacterial infection. Furthermore, rOnGal-2 administration could increase the relative percentage survival of tilapia infected with S.agalactiae. Taken together, our results indicate that OnGal-2 can protect fish from bacterial infection through reducing bacterial burden, impairing tissue damage and modulating anti-bacterial immune response, which also can be applied as a potential vaccine adjuvant in O.niloticus culture.
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Authors | Jinzhong Niu, Xinchao Liu, Zhiqiang Zhang, Yu Huang, Jufen Tang, Bei Wang, Yishan Lu, Jia Cai, Jichang Jian |
Journal | Fish & shellfish immunology
(Fish Shellfish Immunol)
Vol. 106
Pg. 473-479
(Nov 2020)
ISSN: 1095-9947 [Electronic] England |
PMID | 32805415
(Publication Type: Journal Article)
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Copyright | Copyright © 2020 Elsevier Ltd. All rights reserved. |
Chemical References |
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Topics |
- Animals
- Cichlids
(genetics, immunology)
- Fish Diseases
(immunology)
- Fish Proteins
(genetics, immunology)
- Galectin 2
(genetics, immunology)
- Gene Expression Profiling
(veterinary)
- Gene Expression Regulation
(immunology)
- Immunity, Innate
(genetics)
- Male
- Streptococcal Infections
(immunology, veterinary)
- Streptococcus agalactiae
(physiology)
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