Abstract | BACKGROUND: METHODS: The patient received 40 intravenous doses of a single phage (KpJH46Φ2) targeting his bacterial isolate, alongside continued minocycline (which he had been receiving when he developed increasing pain, swelling, and erythema prior to initiation of phage therapy). Serial cytokine and biomarker measurements were performed before, during, and after treatment. The in vitro anti-biofilm activity of KpJH46Φ2, minocycline and the combination thereof was evaluated against a preformed biofilm of the patient's isolate and determined by safranin staining. RESULTS:
Phage therapy resulted in resolution of local symptoms and signs of infection and recovery of function. The patient did not experience treatment-related adverse effects and remained asymptomatic 34 weeks after completing treatment while still receiving minocycline. A trend in biofilm biomass reduction was noted 22 hours after exposure to KpJH46Φ2 (P = .063). The addition of phage was associated with a satisfactory outcome in this case of intractable biofilm-associated prosthetic knee infection. Pending further studies to assess its efficacy and safety, phage therapy holds promise for treatment of device-associated infections.
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Authors | Edison J Cano, Katherine M Caflisch, Paul L Bollyky, Jonas D Van Belleghem, Robin Patel, Joseph Fackler, Michael J Brownstein, Bri'Anna Horne, Biswajit Biswas, Matthew Henry, Francisco Malagon, David G Lewallen, Gina A Suh |
Journal | Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
(Clin Infect Dis)
Vol. 73
Issue 1
Pg. e144-e151
(07 01 2021)
ISSN: 1537-6591 [Electronic] United States |
PMID | 32699879
(Publication Type: Case Reports, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Copyright | © The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: [email protected]. |
Chemical References |
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Topics |
- Anti-Bacterial Agents
(therapeutic use)
- Arthroplasty, Replacement, Knee
(adverse effects)
- Biofilms
- Humans
- Klebsiella pneumoniae
- Male
- Middle Aged
- Phage Therapy
- Prosthesis-Related Infections
(drug therapy)
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