Abstract | INTRODUCTION: METHODS: In this study, adult male Wistar rats (220-250 g) underwent Spinal Nerve Ligation (SNL) surgery. Mechanical allodynia and thermal hyperalgesia were assessed before the surgery (day 0); then, every other day up to day 8. GSK-3β selective inhibitor, AR-014418 [0.3 mg/kg, Intraperitoneal (IP)] was administrated 1 h prior to SNL on day 0, then daily up to the day 8. The GSK-3β activity and apoptosis in the lumbar section (L4, L5, or L6) of the study rat's spinal cord were assessed by immunohistochemical and Terminal Deoxynucleotidyl Transferase dUTP Nick End Labeling (TUNEL) staining, respectively on day 8 post-SNL. RESULTS: CONCLUSION: The study findings suggested that increasing the p-GSK-3β/t-GSK-3β ratio might be a helpful strategy for reducing the apoptotic cells and subsequent neuropathic pain during peripheral nerve injury.
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Authors | Mina Rashvand, Samira Danyali, Homa Manaheji |
Journal | Basic and clinical neuroscience
(Basic Clin Neurosci)
2020 Jan-Feb
Vol. 11
Issue 1
Pg. 15-30
ISSN: 2008-126X [Print] Iran |
PMID | 32483472
(Publication Type: Journal Article)
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Copyright | Copyright© 2020 Iranian Neuroscience Society. |