Phospholipids modifications in human hepatoma cell lines (HepG2) exposed to silver and iron oxide nanoparticles.

Abstract

Metallic nanoparticles such as silver (Ag NPs) and iron oxide (Fe3O4 NPs) nanoparticles are high production volume materials due to their applications in various consumer products, and in nanomedicine. However, their inherent toxicities to human cells remain a challenge. The present study was aimed at combining lipidomics data with common phenotypically-based toxicological assays to gain better understanding into cellular response to Ag NPs and Fe3O4 NPs exposure. HepG2 cells were exposed to different concentrations (3.125, 6.25, 12.5, 25, 50 and 100 µg/ml) of the nanoparticles for 24 h, after which they were assayed for toxic effects using toxicological assays like cytotoxicity, mutagenicity, apoptosis and oxidative stress. The cell membrane phospholipid profile of the cells was also performed using shotgun tandem mass spectrometry. The results showed that nanoparticles exposure resulted in concentration-dependent cytotoxicity as well as reduced cytokinesis-block proliferation index (CBPI). Also, there was an increase in the production of ROS and superoxide anions in exposed cells compared to the negative control. The lipidomics data revealed that nanoparticles exposure caused a modulation of the phospholipidome of the cells. A total of 155 lipid species were identified, out of which the fold changes of 23 were significant. The high number of differentially changed phosphatidylcholine species could be an indication that inflammation is one of the major mechanisms of toxicity of the nanoparticles to the cells.

• Authors: Joseph A Adeyemi, Carlos Arterio Sorgi, Ana Rita Thomazela Machado, Abayomi T Ogunjimi, Luiz Gustavo Araujo Gardinassi, Viviani Nardini, Lucia Helena Faccioli, Lusania Maria Greggi Antunes, Fernando Barbosa Jr
• Journal: Archives of toxicology (Arch Toxicol) Vol. 94 Issue 8 Pg. 2625-2636 (08 2020) ISSN: 1432-0738 [Electronic] Germany
• PMID: 32474618 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Phospholipids
• Silver Compounds
• Superoxides

Topics

• Apoptosis (drug effects)
• Cell Proliferation (drug effects)
• Cytokinesis (drug effects)
• Dose-Response Relationship, Drug
• Hep G2 Cells
• Hepatocytes (drug effects, metabolism, pathology)
• Humans
• Lipidomics
• Magnetic Iron Oxide Nanoparticles (toxicity)
• Metal Nanoparticles (toxicity)
• Necrosis
• Oxidative Stress (drug effects)
• Phospholipids (metabolism)
• Silver Compounds (toxicity)
• Spectrometry, Mass, Electrospray Ionization
• Superoxides (metabolism)
• Tandem Mass Spectrometry