Lancemaside A Isolated from the Root of Codonopsis lanceolata Inhibits Ovarian Cancer Cell Invasion via the Reactive Oxygen Species (ROS)-Mediated p38 Pathway.

Abstract

Codonopsis lanceolata roots have been widely used in Korean cuisine and traditional medicine. This study aimed to investigate the antimetastatic effects of lancemaside A, a major triterpenoid saponin, isolated from the roots of C. lanceolata, in human ovarian cancer cells. Lancemaside A significantly suppressed the migration and invasion and the expression of matrix metalloproteinases (MMPs)-2 and -9 in ovarian cancer A2780 and SKOV3 cells. Treatment with lancemaside A generated reactive oxygen species (ROS) in ovarian cancer cells. However, treatment with anti-oxidant N-acetyl-L-cysteine (NAC) significantly negated the anti-invasive activity of lancemaside A. Additionally, lancemaside A activated p38 MAP kinase, which is mediated by ROS generation. This is the first study, to our knowledge, to reveal that lancemaside A isolated from the roots of C. lanceolata exerts antimetastatic activity through inhibition of MMP expression and cancer cell invasion via activation of the ROS-mediated p38 pathway.

• Authors: Ji-Hye Ahn, Dae Sik Jang, Jung-Hye Choi
• Journal: The American journal of Chinese medicine (Am J Chin Med) Vol. 48 Issue 4 Pg. 1021-1034 ( 2020) ISSN: 1793-6853 [Electronic] Singapore
• PMID: 32471314 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents, Phytogenic
• Reactive Oxygen Species
• Saponins
• lancemaside A
• p38 Mitogen-Activated Protein Kinases

Topics

• Antineoplastic Agents, Phytogenic
• Cell Line, Tumor
• Codonopsis (chemistry)
• Female
• Gene Expression
• Humans
• MAP Kinase Signaling System (drug effects, genetics)
• Neoplasm Invasiveness (genetics, pathology)
• Ovarian Neoplasms (pathology)
• Plant Roots (chemistry)
• Reactive Oxygen Species (metabolism)
• Saponins (isolation & purification, pharmacology)
• p38 Mitogen-Activated Protein Kinases (genetics, metabolism)