Laminarin Pretreatment Provides Neuroprotection against Forebrain Ischemia/Reperfusion Injury by Reducing Oxidative Stress and Neuroinflammation in Aged Gerbils.

Abstract

Laminarin is a polysaccharide isolated from brown algae that has various biological and pharmacological activities, such as antioxidant and anti-inflammatory properties. We recently reported that pretreated laminarin exerted neuroprotection against transient forebrain ischemia/reperfusion (IR) injury when we pretreated with 50 mg/kg of laminarin once a day for seven days in adult gerbils. However, there have been no studies regarding a neuroprotective effect of pretreated laminarin against IR injury in aged animals and its related mechanisms. Therefore, in this study, we intraperitoneally inject laminarin (50 mg/kg) once a day to aged gerbils for seven days before IR (5-min transient ischemia) surgery and examine the neuroprotective effect of laminarin treatment and the mechanisms in the gerbil hippocampus. IR injury in vehicle-treated gerbils causes loss (death) of pyramidal neurons in the hippocampal CA1 field at five days post-IR. Pretreatment with laminarin effectively protects the CA1 pyramidal neurons from IR injury. Regarding the laminarin-treated gerbils, production of superoxide anions, 4-hydroxy-2-nonenal expression and pro-inflammatory cytokines [interleukin(IL)-1β and tumor necrosis factor-α] expressions are significantly decreased in the CA1 pyramidal neurons after IR. Additionally, laminarin treatment significantly increases expressions of superoxide dismutase and anti-inflammatory cytokines (IL-4 and IL-13) in the CA1 pyramidal neurons before and after IR. Taken together, these findings indicate that laminarin can protect neurons from ischemic brain injury in an aged population by attenuating IR-induced oxidative stress and neuroinflammation.

• Authors: Joon Ha Park, Ji Hyeon Ahn, Tae-Kyeong Lee, Cheol Woo Park, Bora Kim, Jae-Chul Lee, Dae Won Kim, Myoung Cheol Shin, Jun Hwi Cho, Choong-Hyun Lee, Soo Young Choi, Moo-Ho Won
• Journal: Marine drugs (Mar Drugs) Vol. 18 Issue 4 (Apr 15 2020) ISSN: 1660-3397 [Electronic] Switzerland
• PMID: 32326571 (Publication Type: Journal Article)

Chemical References

• Cytokines
• Glucans
• Nerve Tissue Proteins
• Neuroprotective Agents
• laminaran
• Superoxide Dismutase

Topics

• Animals
• Brain Ischemia (drug therapy)
• Cytokines (metabolism)
• Disease Models, Animal
• Gerbillinae
• Glucans (pharmacology)
• Hippocampus (drug effects)
• Inflammation (metabolism)
• Male
• Nerve Tissue Proteins
• Neurons (drug effects, metabolism)
• Neuroprotection
• Neuroprotective Agents (pharmacology)
• Oxidative Stress (drug effects)
• Reperfusion Injury (drug therapy)
• Superoxide Dismutase (metabolism)