Abstract | BACKGROUND: Micronuclei (MN), nuclear bud (NBud), and nucleoplasmic bridge ( NPB) are suggested as biomarkers for radiation exposure; however, they have not been extensively studied to understand the underlying mechanisms responsible for their formation. OBJECTIVES: To (1) validate NBud and NPB within the cytokinesis-blocked micronucleus (CBMN) assay as biomarkers for radiation exposure and (2) determine the effects of the DNA repair inhibitors, cytosine arabinoside ( Ara C) and 3-aminobenzamide (3-AB) on radiation-induced MN, NBud, and NPB formation. METHODS: Human blood samples were irradiated with 0-3 Gy X-rays and subsequently treated with Ara C and 3-AB. CBMN and chromosome aberration assays were carried out to measure MN, NBud, and NPB and dicentric chromosomes, respectively. RESULTS: The frequency of radiation-induced MN, NBud, and NPB increased in a dose-dependent manner. The frequency of MN, NBud, and NPB was highly and positively correlated with the dicentric chromosome, a standard biomarker for biodosimetry (r > 0.98, p < 0.0001). Furthermore, Ara C increased the frequency of MN, NBud, and NPB, whereas 3-AB increased the frequency of MN and NPB, but not NBud. Further, the potentiating effect of Ara C on the frequency of MN, NBud, and NPB was greater than that of 3-AB. CONCLUSION: Our results validate NBuds and NPBs as independent valuable markers of radiation exposure. Additionally, we suggest that different mechanisms are likely involved in the formation of NBuds and NPBs following X-irradiation; however, additional studies are warranted to better understand the contribution of distinct DNA repair pathways to the formation of radiation-induced damages.
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Authors | Yoon Hee Cho, Yang Jee Kim, Sunyeong Lee, Kyung-In Joung, Hai Won Chung, Sunmi Kim, Su Young Kim |
Journal | Genes & genomics
(Genes Genomics)
Vol. 42
Issue 6
Pg. 673-680
(06 2020)
ISSN: 2092-9293 [Electronic] Korea (South) |
PMID | 32314273
(Publication Type: Journal Article)
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Chemical References |
- Benzamides
- Cytarabine
- 3-aminobenzamide
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Topics |
- Adult
- Benzamides
(pharmacology)
- Cells, Cultured
- Cytarabine
(pharmacology)
- DNA Repair
(drug effects)
- Female
- Humans
- Micronuclei, Chromosome-Defective
(drug effects, radiation effects)
- Radiation Tolerance
- X-Rays
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