Abstract |
Context: A growing body of evidence demonstrates that gastrointestinal motility disorder (GIMD) and gastric stress ulcers can be induced by restraint stress, while melatonin (MT) elicits anti- inflammation and antioxidant effects.Objective: The present study investigated the mechanisms of MT-mediated protection effects on restraint stress-induced GIMD.Materials and methods: 144 8-week-old male ICR mice were divided into four groups: control, restraint stress, restraint stress + MT and MT (positive control). 20 mg/kg MT or vehicle were intraperitoneally injected 60 min before restraint stress (10 h/day) once daily for 3 days. Biochemical parameters, intestinal mucosal integrity, tissues antioxidant ability and autophagic proteins levels were determined.Results: Mice subjected to restraint stress elevated NE level by 141.41% and decreased MT content by 38.82% in plasma. Consistent with the decrease in MT level, we observed a reduction in the antioxidant ability and an increase in autophagic proteins by 14.29-46.74% in the gut, resulting in injury to intestinal mucosa which was manifested by reductions in villus height and villus height/crypt depth (V/C) ratio, number of goblet and PCAN-positive cells, and expression of tight junction protein (ZO-1, occludin and claudin-1). In contrast, MT reversed these changes caused by restraint stress and improved the intestinal mucosal injury. However, there was no significant difference between MT (positive control) and control group.Discussion and conclusion: Our results suggest that MT effectively mitigates psychological stress-induced injury to intestinal mucosa, providing evidence demonstrating the potential for using MT as therapy against intestinal impairment associated with psychological stress.
|
Authors | Rutao Lin, Zixu Wang, Jing Cao, Ting Gao, Yulan Dong, Yaoxing Chen |
Journal | Pharmaceutical biology
(Pharm Biol)
Vol. 58
Issue 1
Pg. 342-351
(Dec 2020)
ISSN: 1744-5116 [Electronic] England |
PMID | 32298156
(Publication Type: Journal Article)
|
Chemical References |
- Anti-Inflammatory Agents
- Antioxidants
- Tight Junction Proteins
- Phenylpropanolamine
- Melatonin
|
Topics |
- Animals
- Anti-Inflammatory Agents
(administration & dosage, pharmacology, therapeutic use)
- Antioxidants
(administration & dosage, pharmacology, therapeutic use)
- Autophagy
(drug effects)
- Gastrointestinal Diseases
(drug therapy, etiology)
- Intestinal Mucosa
(cytology, drug effects, metabolism, pathology)
- Male
- Melatonin
(administration & dosage, pharmacology, therapeutic use)
- Mice
- Mice, Inbred ICR
- Phenylpropanolamine
(metabolism)
- Restraint, Physical
- Stress, Psychological
(complications)
- Tight Junction Proteins
(metabolism)
|