Lonicera caerulea L. Polyphenols Alleviate Oxidative Stress-Induced Intestinal Environment Imbalance and Lipopolysaccharide-Induced Liver Injury in HFD-Fed Rats by Regulating the Nrf2/HO-1/NQO1 and MAPK Pathways.

Abstract

SCOPE:

This study investigates the modulatory effects of Lonicera caerulea L. polyphenols (LCPs) on the intestinal environment and lipopolysaccharide (LPS)-induced liver injury via the nuclear factor erythroid-2-related factor 2/heme oxygenase-1 (HO-1)/NQO1 and mitogen-activated protein kinase (MAPK) pathways in a rat model of oxidative stress damage (OSD).

METHODS AND RESULTS:

To examine the prebiotic properties of LCP, a model of high-fat-diet-induced OSD is established using Sprague Dawley rats. In the colon, treatment with LCP for 8 weeks ameliorates enhanced intestinal permeability (glucagon-like peptide-2 content and occludin protein increase, whereas claudin-2 protein decreases), intestinal inflammation (levels of pro-inflammatory cytokines, such as tumor necrosis factor-α, interleukin-6, cyclooxygenase-2, and nuclear factor kappa-B p65 (NF-κB p65), decrease), and intestinal OSD (through regulation of the Nrf2/HO-1/NQO1 pathway). Moreover, LCP alleviates LPS-induced liver injury by suppressing the nuclear translocation of NF-κB p65 and activation of the MAPK signaling pathway. Additionally, Bacilli, Lactobacillales, Lactobacillaceae, Lactobacillus, Akkermansia, Actinobacteria, Proteobacteria, Rothia, and Blautia are found to be the key intestinal microbial taxa related to intestinal OSD and LPS-induced liver injury in rats.

CONCLUSION:

LCP treatment potentially modulates the intestinal environment and alleviates liver injury by suppressing oxidative-stress-related pathways and altering the composition of the intestinal microbiota.

• Authors: Bin Li, Zhen Cheng, Xiyun Sun, Xu Si, Ersheng Gong, Yuehua Wang, Jinlong Tian, Chi Shu, Fengming Ma, Dongnan Li, Xianjun Meng
• Journal: Molecular nutrition & food research (Mol Nutr Food Res) Vol. 64 Issue 10 Pg. e1901315 (05 2020) ISSN: 1613-4133 [Electronic] Germany
• PMID: 32250024 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Lipopolysaccharides
• NF-E2-Related Factor 2
• Nfe2l2 protein, rat
• Polyphenols
• Heme Oxygenase (Decyclizing)
• Hmox1 protein, rat
• NAD(P)H Dehydrogenase (Quinone)
• NQO1 protein, rat

Topics

• Animals
• Body Weight (drug effects)
• Colon (drug effects, pathology)
• Diet, High-Fat (adverse effects)
• Gastrointestinal Microbiome (drug effects)
• Gene Expression Regulation (drug effects)
• Heme Oxygenase (Decyclizing) (metabolism)
• Lipopolysaccharides (toxicity)
• Liver (drug effects, metabolism, pathology)
• Lonicera (chemistry)
• MAP Kinase Signaling System (drug effects)
• Male
• NAD(P)H Dehydrogenase (Quinone) (metabolism)
• NF-E2-Related Factor 2 (metabolism)
• Oxidative Stress (drug effects)
• Polyphenols (pharmacology)
• Rats, Sprague-Dawley