Bioactive borate glass triggers phenotypic changes in adipose stem cells.

Abstract

A bioactive borate glass, 13-93B3 (B3), has been used successfully in the clinic to treat chronic, nonhealing wounds without scarring. However, the mechanism by which B3 stimulates wound healing is poorly understood. Because adipose stem cells (ASCs) have been shown to have multiple roles in wound repair, we hypothesized that B3 triggers ASCs. In this study, we evaluate the effects of B3 on ASC survival, migration, differentiation, and protein secretion in vitro. In concentrations ≤10 mg/ml, B3 did not affect ASC viability under static conditions. B3 promoted the migration of ASCs but did not increase differentiation into bone or fat. B3 also decreased ASCs secretion of collagen I, PAI-1, MCP-1, DR6, DKK-1, angiogenin, IL-1, IGFBP-6, VEGF, and TIMP-2; increased expression of IL-1R and E-selectin; had a transient decrease in IL-6 secretion; and had a transient increase in bFGF secretion. Together, these results show that B3 alters the protein secretion of ASCs.

• Authors: Nathan J Thyparambil, Lisa C Gutgesell, Bradley A Bromet, Lauren E Flowers, Samantha Greaney, Delbert E Day, Julie A Semon
• Journal: Journal of materials science. Materials in medicine (J Mater Sci Mater Med) Vol. 31 Issue 4 Pg. 35 (Mar 23 2020) ISSN: 1573-4838 [Electronic] United States
• PMID: 32206916 (Publication Type: Journal Article)

Chemical References

• Biocompatible Materials
• Borates

Topics

• Adipose Tissue (cytology)
• Biocompatible Materials
• Borates (chemistry)
• Cell Differentiation
• Cell Movement
• Cell Survival
• Gene Expression Regulation
• Glass (chemistry)
• Humans
• Materials Testing
• Stem Cells (drug effects)