Anti-fibrotic activity of gold and platinum complexes - Au(I) compounds as a new class of anti-fibrotic agents.

Abstract

Molecular gold(I) and platinum(II) species were examined for the inhibition of liver fibrosis and the hepatitis C virus (HCV). Determination of inhibition efficiency was conducted via morphological analysis, cell viability, western blot analysis, and quantitative reverse transcription polymerase chain reaction (RT-PCR). Auranofin and Ph3PAuCl demonstrated the greatest inhibition of liver fibrosis amongst the tested gold species in human hepatic stellate LX-2 cells. Western blot analysis indicated that auranofin and Ph3PAuCl prevent signal transducer and activator of transcription 3 (STAT3) phosphorylation, which may be a key connection to fibrosis and inflammation. Auranofin and Ph3PAuCl also reduced expression of HCV-nonstructural protein 3 (NS3) and HCV-NS5a proteins in a HCV subgenomic replicon system. These results demonstrate significant promise for the use of gold compounds in treating liver diseases such as HCV.

• Authors: Matthew Stratton, Akshaya Ramachandran, Ehxciquiel Jaeroume M Camacho, Shivaputra Patil, Gulam Waris, Kyle A Grice
• Journal: Journal of inorganic biochemistry (J Inorg Biochem) Vol. 206 Pg. 111023 (05 2020) ISSN: 1873-3344 [Electronic] United States
• PMID: 32163811 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2020 Elsevier Inc. All rights reserved.

Chemical References

• Organogold Compounds
• Organoplatinum Compounds
• Platinum Compounds
• STAT3 Transcription Factor
• Auranofin
• Platinum
• Gold

Topics

• Auranofin (pharmacology)
• Cell Line
• Cell Survival
• Gold (chemistry)
• Hepacivirus (metabolism)
• Hepatitis C (drug therapy, metabolism, pathology)
• Humans
• Liver Cirrhosis (drug therapy, metabolism, pathology)
• Organogold Compounds (chemistry, pharmacology)
• Organoplatinum Compounds (chemistry, pharmacology)
• Phosphorylation
• Platinum (chemistry)
• Platinum Compounds (chemistry, pharmacology)
• STAT3 Transcription Factor (metabolism)