Abstract |
Sirtuin 6 (Sirt6) is predominantly expressed in epithelial cells in intestinal crypts. It plays an important role in protecting intestinal epithelial cells against inflammatory injury. Previously, we found that colitis is associated with the downregulation of Sirt6 protein in the intestines. Here, we report that murine interferon-γ (Ifnγ) inhibits Sirt6 protein but not mRNA expression in young adult mouse colonocytes (YAMC, a mouse colonic epithelial cell line) in a dose- and time-dependent manner. Using microRNA array analysis, we showed that Ifnγ induces expression of miR-92b in YAMC cells. With in silico analysis, we found that the Sirt6 3'-untranslated region (UTR) contains a putative binding site for miR-92b. Luciferase assay showed that Ifnγ inhibited Sirt6 3'-UTR activity and this effect was mimicked by miR-92b via directly targeting the miR-92b seed site in the 3'-UTR of Sirt6 mRNA. Furthermore, Western blot demonstrated that miR-92b downregulated Sirt6 protein expression in YAMC cells. Blocking miR-92b with a specific inhibitor attenuated the inhibitory effect of Ifnγ on Sirt6 protein expression in the cells. Collectively, our data suggest that Ifnγ inhibits Sirt6 protein expression in intestinal epithelial cells via a miR-92b-mediated mechanism. miR-92b may be a novel therapeutic target for rescuing Sirt6 protein levels in intestinal epithelial cells, thereby protecting against intestinal mucosal injury caused by inflammation.
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Authors | Fangyi Liu, Xiao Wang, Hua Geng, Heng-Fu Bu, Peng Wang, Isabelle G De Plaen, Hong Yang, Jiaming Qian, Xiao-Di Tan |
Journal | American journal of physiology. Cell physiology
(Am J Physiol Cell Physiol)
Vol. 318
Issue 4
Pg. C732-C739
(04 01 2020)
ISSN: 1522-1563 [Electronic] United States |
PMID | 32049548
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- MicroRNAs
- Interferon-gamma
- Sirt6 protein, mouse
- Sirtuins
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Topics |
- Animals
- Cell Line
- Epithelial Cells
(drug effects, metabolism)
- Gene Expression
(drug effects)
- Inflammation
(drug therapy, genetics, metabolism)
- Interferon-gamma
(metabolism, pharmacology)
- Intestinal Mucosa
(metabolism)
- Intestines
(drug effects)
- MicroRNAs
(genetics)
- Sirtuins
(genetics, metabolism)
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