Abstract | BACKGROUND/AIMS: METHODS: RT-qPCR was applied to study the expression level of ARPP19 and miR-802 in the laryngeal carcinoma cell lines and tissues. CCK-8, colony formation, flow cytometry (FACS) assay were used to study the effect of ARPP19 and miR-802 on apoptosis, proliferation, and cell cycle of laryngeal carcinoma cells. Target gene prediction and luciferase reporter gene assay were applied to identify target gene of miR-802. The transcriptional mRNA and protein expression levels of ARPP19 were measured by RT-qPCR or Western blotting. RESULTS: miR-802 was down-regulated in laryngeal carcinoma cell lines and tissues. Laryngeal cancer cells transfected by miR-802 mimic were significantly inhibited in the terms of cell colony formation and proliferation. Furthermore, miR-802 can inhibit the expression level of ARPP19 by directly targeting the 3' untranslated region (3'-UTR) of ARPP19. Overexpression of the ARPP19 gene can reverse the suppressive effect of miR-802 on laryngeal cancer cells. CONCLUSION:
|
Authors | Huafu Ye, Qiaozhi Jin, Xiaoqiong Wang, Yong Li |
Journal | Cancer management and research
(Cancer Manag Res)
Vol. 12
Pg. 419-430
( 2020)
ISSN: 1179-1322 [Print] New Zealand |
PMID | 32021454
(Publication Type: Journal Article, Retracted Publication)
|
Copyright | © 2020 Ye et al. |