Lipopolysaccharide stimulates the intestinal microbiome to activate
phosphoinositide 3 kinase (PI3K) signaling via several pathways; however, the direct effect that PI3K has on the intestinal bacterial community remains unclear. Herein, we investigate changes in the colonic microbiome of
colitis PI3Kγ-knockout (PI3Kγ-/-) mice. Additionally, the effect of anal administration of colonic irrigation fluid from control mice to those with
colitis was examined. Microbial
16S rRNA genes from the colonic mucosa of PI3Kγ-/- and WT mice were sequenced using Illumina MiSeq platform, and colonic
IgA,
IL-2,
IL-10, and
IL-17A production was quantified by western blot analysis.
Myeloperoxidase (MPO) activity was detected by absorbance via colorimetric analysis. From the results, two new indices were derived by dividing the bacterial community into invading taxa, common taxa, and vanishing taxa. These indices were used to estimate the degree of microbiome disorder in chronic experimental
colitis models. PI3Kγ-/- mice showed slower remission of
inflammation as assessed by the disease activity index,pathological score,
IL-2,
IL-17,
IL-10,
IgA expression and MPO activity. The unique and common taxa of wild-type and PI3Kγ-/- mice increased as
colitis symptoms regressed. Continuous loss of commensal bacteria happened with the continuous invasion of exogenous bacteria in the intestinal mucosa of PI3Kγ--/- mice after
colitis begin to aggravate. However,
transplantation of normal intestinal microbiota to PI3Kγ-/- mice promoted remission of
inflammation; while the microbial
dysbiosis observed during PI3Kγ dysfunction aggravated the intestinal microbiome disorder and impeded
colitis recovery. Thus, the PI3Kγ signaling pathway may regulate microbial community composition in the colon.