LncRNA DLEU1 accelerates the malignant progression of clear cell renal cell carcinoma via regulating miRNA-194-5p.

Abstract

OBJECTIVE:

The aim of this study was to illustrate the role of long non-coding RNA (lncRNA) DLEU1 in regulating the malignant progression of clear cell renal cell carcinoma (ccRCC) by targeting microRNA-194-5p (miRNA-194-5p).

PATIENTS AND METHODS:

DLEU1 expression level in ccRCC tissues and para-cancerous tissues was determined by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The correlation between DLEU1 expression and pathological indexes of ccRCC patients was assessed. After the silence of DLUE1, the proliferative and migratory abilities of ACHN and 786-O cells were evaluated. Furthermore, Dual-Luciferase reporter gene assay and rescue experiments were conducted to identify the role of DLEU1/miRNA-194-5p in regulating the ccRCC progression in vitro.

RESULTS:

DLEU1 expression was markedly up-regulated in ccRCC tissues when compared with para-cancerous tissues. The rates of lymphatic metastasis and distant metastasis in ccRCC patients with a high level of DLEU1 were significantly higher, whereas the prognosis was significantly worse. Transfection of si-DLEU1 remarkably attenuated proliferative and migratory abilities of ACHN and 786-O cells. MiRNA-194-5p was identified as the target gene of DLEU1. In addition, the knockdown of miRNA-194-5p could reverse the regulatory effect of DLEU1 on the proliferative and metastatic abilities of ccRCC.

CONCLUSIONS:

DLEU1 is closely related to lymphatic metastasis, distant metastasis, and poor prognosis of ccRCC. It aggravates the progression of ccRCC by targeting miRNA-194-5p.

• Authors: G-Z He, S-Y Yu, Q-P Zhou, M-L Chen, Y-W Zhang, Y Zheng, Z-B Zhang, Z-Y Han, J Yu
• Journal: European review for medical and pharmacological sciences (Eur Rev Med Pharmacol Sci) Vol. 23 Issue 24 Pg. 10691-10698 (Dec 2019) ISSN: 2284-0729 [Electronic] Italy
• PMID: 31858537 (Publication Type: Journal Article, Retracted Publication)

Chemical References

• DLEU1 lncRNA, human
• MIRN194 microRNA, human
• MicroRNAs
• RNA, Long Noncoding
• Tumor Suppressor Proteins

Topics

• Aged
• Carcinoma, Renal Cell (genetics, pathology)
• Cell Line, Tumor
• Cell Movement (genetics)
• Cell Proliferation (genetics)
• Female
• Gene Silencing
• Humans
• Kidney Neoplasms (genetics, pathology)
• Male
• MicroRNAs (genetics)
• Middle Aged
• Prognosis
• RNA, Long Noncoding (genetics)
• Transfection
• Tumor Suppressor Proteins (genetics)
• Up-Regulation