Vaccination of mice with a recombinant novel cathepsin B inhibits Trichinella spiralis development, reduces the fecundity and worm burden.

Abstract

BACKGROUND:

Trichinella spiralis is a major zoonotic tissue-dwelling nematode, which is a public health concern and a serious hazard to animal food safety. It is necessary to exploit an anti-Trichinella vaccine to interrupt the transmission of Trichinella infection among animals and from animals to humans. The purpose of the present study was to characterize the novel T. spiralis cathepsin B (TsCB) and to evaluate the immune protection elicited by immunization with recombinant TsCB (rTsCB).

METHODS:

The complete cDNA sequences of the TsCB gene were cloned, expressed and purified. The antigenicity of rTsCB was investigated by western blot analysis and ELISA. Transcription and expression of TsCB at various T. spiralis life-cycle stages were analyzed by RT-PCR and indirect immunofluorescent assay (IIFA). The mice were subcutaneously immunized with rTsCB, and serum level of TsCB-specific IgG (IgG1 and IgG2a) and IgE antibodies were assayed by ELISA. Immune protection elicited by vaccination with rTsCB was investigated.

RESULTS:

The TsCB was transcribed and expressed in four T. spiralis life-cycle stages (adult worm, AW; newborn larvae, NBL; muscle larvae, ML; and intestinal infective L1 larvae), it was primarily located in the cuticle and stichosome of the parasitic nematode. Vaccination of mice with rTsCB produced a prominent antibody response (high level of specific IgG and IgE) and immune protection, as demonstrated by a 52.81% AW burden reduction of intestines at six days post-infection (dpi) and a 50.90% ML burden reduction of muscles at 35 dpi after oral larva challenge. The TsCB-specific antibody response elicited by immunization with rTsCB also impeded intestinal worm growth and decreased the female fecundity.

CONCLUSIONS:

TsCB might be considered as a novel potential molecular target to develop vaccines against T. spiralis infection.

• Authors: Jing Cui, Yue Han, Xin Yue, Fang Liu, Yan Yan Song, Shu Wei Yan, Jun Jun Lei, Xi Zhang, Peng Jiang, Zhong Quan Wang
• Journal: Parasites & vectors (Parasit Vectors) Vol. 12 Issue 1 Pg. 581 (Dec 11 2019) ISSN: 1756-3305 [Electronic] England
• PMID: 31829230 (Publication Type: Journal Article)

Chemical References

• Antibodies, Helminth
• Antigens, Helminth
• Immunoglobulin G
• Vaccines, Synthetic
• Immunoglobulin E
• Cathepsin B

Topics

• Animals
• Antibodies, Helminth (blood)
• Antigens, Helminth (administration & dosage, immunology)
• Cathepsin B (administration & dosage, immunology)
• Disease Models, Animal
• Enzyme-Linked Immunosorbent Assay
• Fertility
• Immunoglobulin E (blood)
• Immunoglobulin G (blood)
• Injections, Subcutaneous
• Mice
• Parasite Load
• Treatment Outcome
• Trichinella spiralis (growth & development, immunology, isolation & purification)
• Trichinellosis (parasitology, pathology, prevention & control)
• Vaccines, Synthetic (administration & dosage, immunology)