2-OHOA supplementation reduced adiposity and improved cardiometabolic risk to a greater extent than n-3 PUFA in obese mice.

Abstract

OBJECTIVE:

We aimed to assess whether 2-hydroxyoleic acid (2-OHOA) and n-3 polyunsaturated fatty acids (PUFA) could counteract changes on adipokine secretion and cardiometabolic risk biomarkers associated with high-fat diet-induced obesity in mice.

METHODS:

Female ICR/CD1 mice (8 weeks old) were divided into four groups receiving different diets (n=8/group): (1) standard chow (control) for 18 weeks; (2) 22% fat for 4 weeks + 60% fat for 14 weeks (obesogenic diet, OD); 3) OD + 2-OHOA (1500mgkg-1 diet) for the last 6 weeks (ODHO); and 4) OD+n-3 PUFA (eicosapentaenoic+docosahexaenoic acids, 1500+1500mgkg-1 diet) for the last 6 weeks (OD-N3). After 18 weeks, body weight, periovarian visceral fat, heart and liver weights were measured, as well as cardiometabolic parameters (systolic and diastolic blood pressure, blood glucose, insulin, HOMA index, triglycerides, total cholesterol, apolipoproteins A1 and E), plasma adipokines and inflammatory proteins (leptin, adiponectin, plasminogen activator inhibitor 1 [PAI1], soluble E-selectin [sE-selectin], matrix metalloproteinase-9 [MMP-9], fibrinogen, soluble intercellular adhesion molecule [sICAM] and soluble vascular adhesion molecule [sVCAM]), and secretion of pro-inflamatory cytokines and inflammatory biomarkers from periovarian adipocytes.

RESULTS:

OD mice had greater body and heart weights, and plasma leptin, and lower adiponectin and resistin secretion from adipocytes. Supplementation with 2-OHOA reduced body and heart weights, blood pressure, triglycerides and leptin, and restored adiponectin and resistin secretion, while n-3 PUFA only reduced triglyceride levels (all P<0.05).

CONCLUSION:

2-OHOA supplementation was more effective in reducing adiposity, modulating adipokine secretion and ameliorating cardiometabolic risk than n-3 PUFA.

• Authors: Noemí Redondo Useros, Alina Gheorghe, Fátima Perez de Heredia, Ligia E Díaz, Gyselle Chrystina Baccan, Mónica De la Fuente, Ascensión Marcos
• Journal: Obesity research & clinical practice (Obes Res Clin Pract) 2019 Nov - Dec Vol. 13 Issue 6 Pg. 579-585 ISSN: 1871-403X [Print] Netherlands
• PMID: 31787558 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2019 Asia Oceania Association for the Study of Obesity. All rights reserved.

Chemical References

• 2-hydroxyoleic acid
• Adiponectin
• Fatty Acids, Omega-3
• Leptin
• Oleic Acids
• Resistin
• Triglycerides

Topics

• Adiponectin (blood)
• Adiposity (drug effects)
• Animals
• Blood Pressure (drug effects)
• Cardiovascular Diseases (blood, prevention & control)
• Dietary Supplements
• Fatty Acids, Omega-3 (pharmacology)
• Female
• Leptin (blood)
• Metabolic Diseases (blood, prevention & control)
• Mice
• Mice, Inbred ICR
• Mice, Obese
• Obesity (blood)
• Oleic Acids (pharmacology)
• Resistin (blood)
• Risk
• Triglycerides (blood)