Abstract |
Mice exposed to the air pollutant ozone develop eosinophilic rhinitis that is mediated by group 2, GATA-3+, innate lymphoid cells (ILC2s). In the present study, we determined the influx, persistence, and recall of nasal ILC2s and eosinophils in ozone-exposed mice. C57BL/6 (T/B cell sufficient, ILC sufficient), Rag2-/- (T/B cell deficient, ILC sufficient), and Rag2-/-Il2rg-/- (T/B cell deficient, ILC deficient) mice were exposed to 0 or 0.8 ppm ozone for 1 or 9 weekdays and killed 1 or 17 days postexposure. GATA-3+ lymphocytes were sparse in nasal tissue of air-exposed ILC-sufficient mice and absent in ILC-deficient mice. Nine-day, but not 1-day, ozone exposures induced nasal influxes of eosinophils and GATA-3+ lymphocytes in C57BL/6 and Rag2-/- mice but not in Rag2-/-Il2rg-/- mice. Eosinophils waned 17 days postexposure in ILC-sufficient strains of mice. GATA-3+ lymphocytes in C57BL/6 mice also attenuated after exposure but not in ILC-sufficient Rag2-/- mice. Eosinophils, but not GATA-3+ cells, increased rapidly with reexposure in ILC-sufficient mice. Type 2 immune-related messenger RNA expression correlated with cellular responses to ozone. These new findings in mice further elucidate the role of ILC2s in ozone-induced eosinophilic rhinitis and support epidemiologic associations between ozone exposure and eosinophilic inflammation in children.
|
Authors | Jack R Harkema, Elyse A Eldridge, Ryan P Lewandowski, James G Wagner |
Journal | Toxicologic pathology
(Toxicol Pathol)
Vol. 48
Issue 2
Pg. 323-337
(02 2020)
ISSN: 1533-1601 [Electronic] United States |
PMID | 31729279
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Air Pollutants
- GATA3 Transcription Factor
- Gata3 protein, mouse
- Oxidants, Photochemical
- Ozone
|
Topics |
- Air Pollutants
(toxicity)
- Animals
- Eosinophils
(immunology)
- GATA3 Transcription Factor
- Lymphocytes
(drug effects, immunology)
- Male
- Mice
- Mice, Inbred C57BL
- Nasal Mucosa
(drug effects, immunology)
- Oxidants, Photochemical
(toxicity)
- Ozone
(toxicity)
- Rhinitis
(chemically induced, immunology)
|