Abstract | BACKGROUND: METHODS: Standardized total protein extracts obtained from wheat seeds were separated by 2D electrophoresis. Twenty-one sera with high wheat-specific immunoglobulin E (sIgE) levels were classified into three patients groups based on their clinical profile. These sera were tested by Western blot on 2D separated standardized wheat protein extract and their sIgE sensitization profiles were compared. RESULTS: Specific sensitization profiles were identified for each phenotype group. For WDEIA, protein spots around 37 kDa (pH 6-9) and 37-50 kDa (pH 5-6) were identified. For AD, spots were observed around 50 kDa (pH 9), 10 kDa (pH 9) and 20 to 75 kDa (pH3). For PR, specific spots were located around 90 kDa (pH 9). The mass spectrometry (UHPLC-MS/MS) analysis of these identified spots pointed out several potential interesting allergens: Tri a 26, Tri a bA, Tri a 34, Tri a tritin. CONCLUSIONS: The present study allowed the identification of different protein areas specific to these studied groups. The protein spots of interest were identified by UHPLC-MS/MS. It has been possible to establish a link between a specific symptomatology and the newly identified responsible allergens.
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Authors | J Courtois, C Bertholet, S Tollenaere, X Van der Brempt, E Cavalier, S El Guendi, N Gillard, R Gadisseur, B Quinting |
Journal | Journal of pharmaceutical and biomedical analysis
(J Pharm Biomed Anal)
Vol. 178
Pg. 112907
(Jan 30 2020)
ISSN: 1873-264X [Electronic] England |
PMID | 31634755
(Publication Type: Journal Article)
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Copyright | Copyright © 2019 Elsevier B.V. All rights reserved. |
Chemical References |
- Allergens
- Immunoglobulin E
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Topics |
- Adolescent
- Adult
- Allergens
(immunology)
- Blotting, Western
- Child
- Child, Preschool
- Chromatography, High Pressure Liquid
- Female
- Humans
- Immunoglobulin E
(immunology)
- Male
- Middle Aged
- Tandem Mass Spectrometry
- Wheat Hypersensitivity
(diagnosis, immunology)
- Young Adult
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