α-Synuclein RT-QuIC assay in cerebrospinal fluid of patients with dementia with Lewy bodies.

Abstract

We applied RT-QuIC assay to detect α-synuclein aggregates in cerebrospinal fluid (CSF) of patients with suspected Creutzfeldt-Jakob disease who had a neuropathological diagnosis of dementia with Lewy bodies (DLB) (n = 7), other neurodegenerative diseases with α-synuclein mixed pathology (n = 20), or without Lewy-related pathology (n = 49). The test had a sensitivity of 92.9% and specificity of 95.9% in distinguishing α-synucleinopathies from non-α-synucleinopathies. When performed in the CSF of patients with DLB (n = 36), RT-QuIC was positive in 17/20 with probable DLB, 0/6 with possible DLB, and 0/10 with Alzheimer disease. These results indicate that RT-QuIC for α-synuclein is an accurate test for DLB diagnosis.

• Authors: Matilde Bongianni, Anna Ladogana, Stefano Capaldi, Sigrid Klotz, Simone Baiardi, Annachiara Cagnin, Daniela Perra, Michele Fiorini, Anna Poleggi, Giuseppe Legname, Tatiana Cattaruzza, Francesco Janes, Massimo Tabaton, Bernardino Ghetti, Salvatore Monaco, Gabor G Kovacs, Piero Parchi, Maurizio Pocchiari, Gianluigi Zanusso
• Journal: Annals of clinical and translational neurology (Ann Clin Transl Neurol) Vol. 6 Issue 10 Pg. 2120-2126 (10 2019) ISSN: 2328-9503 [Electronic] United States
• PMID: 31599499 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: © 2019 University of Verona. Annals of Clinical and Translational Neurology published by Wiley Periodicals, Inc. on behalf of American Neurological Association.

Chemical References

• alpha-Synuclein

Topics

• Alzheimer Disease (cerebrospinal fluid, diagnosis)
• Biological Assay (standards)
• Creutzfeldt-Jakob Syndrome (cerebrospinal fluid, diagnosis)
• Diagnosis, Differential
• Humans
• Lewy Body Disease (cerebrospinal fluid, diagnosis)
• Sensitivity and Specificity
• alpha-Synuclein (cerebrospinal fluid)