Inhibition of Lung Cancer Proliferation by Wogonin is Associated with Activation of Apoptosis and Generation of Reactive Oxygen Species

Abstract

Background:

Lung cancer has a very high incidence rate and is one of the commonly diagnosed tumors in developed countries.

Aims:

To investigate the effect of wogonin on A549 and A427 lung cancer cells and explore the mechanism involved.

Study Design:

Cell study.

Methods:

The cytotoxicity effect of wogonin on A549 and A427 lung cancer and BEAS-2B cells was assessed by MTT assay. The onset of apoptosis was assessed by flow cytometry using Annexin V FITC/PI staining. Western blotting was used for the determination of changes in apoptotic protein expression.

Results:

Wogonin treatment exhibited cytotoxicity effect selectively on A549 and A427 cells without affecting BEAS-2B normal lung cells. The viability of A549 and A427 cells was reduced to 31% and 34%, respectively, on treatment with 50 μM of wogonin; however, there was no significant reduction in BEAS-2B cell viability on treatment with the same concentration of it. Moreover, the percentage of apoptotic A427 cells showed a significant (p<0.049) increase on treatment with wogonin. Furthermore, the treatment led to a marked increase in the activation of caspase 3/8/9 and the generation of reactive oxygen species (ROS) at 72 h in A427 cells. Digital tomosynthesis studies showed a marked reduction in tumor development on treatment with wogonin.

Conclusion:

Wogonin treatment specifically exhibits a cytotoxic effect on lung cancer cells and this effect is associated with activation of apoptosis and generation of reactive oxygen species.

• Authors: Chengyang Wang, Chuangcheng Cui
• Journal: Balkan medical journal (Balkan Med J) Vol. 37 Issue 1 Pg. 29-33 (12 20 2019) ISSN: 2146-3131 [Electronic] Turkey
• PMID: 31594288 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Drugs, Chinese Herbal
• Flavanones
• Reactive Oxygen Species
• wogonin

Topics

• A549 Cells (drug effects)
• Antineoplastic Agents (pharmacology, therapeutic use)
• Apoptosis (drug effects)
• Cell Proliferation (drug effects)
• China
• Drugs, Chinese Herbal (pharmacology, therapeutic use)
• Flavanones (pharmacology, therapeutic use)
• Humans
• Lung Neoplasms (drug therapy, genetics, physiopathology)
• Reactive Oxygen Species