Clinical Aspects of the Dense Fine Speckled Pattern in Indirect Immunofluorescence-Antinuclear Antibody Screening and Its Association with DFS70 Autoantibodies.

Abstract

GOALS:

The existence of anti-dense fine speckled (DFS) 70 autoantibodies are considered an exclusionary biomarker for systemic autoimmune rheumatic diseases (SARDs). Several tests to confirm the presence of anti-DFS70 autoantibodies have been introduced, and the use of them in specimens with a DFS pattern in indirect immunofluorescence-antinuclear antibody (IIF-ANA) testing has been suggested. However, these additional tests have only been evaluated in a small number of samples; their clinical usefulness therefore requires further evaluation.

METHODS:

A total of 213 serum specimens showing DFS (n=155) or homogeneous (H; n=58) patterns were included. All specimens were tested by western blotting (WB) and enzyme immunoassay (EIA). Clinical information regarding SARDs was analyzed.

RESULTS:

The detection rates for WB and EIA for anti-DFS70 autoantibodies in specimens with a DFS pattern were 86.5% and 73.5%, respectively. Detection rates in specimens with a low IIF-ANA titer were significantly lower than those in specimens with a high titer. The detection rate of anti-DFS70 autoantibodies in 58 specimens with an H pattern was 10.3% (6/58). Among 155 subjects with a DFS pattern in IIF-ANA staining, only five were diagnosed with SARD.

CONCLUSIONS:

There is little need to confirm the presence of anti-DFS70 autoantibodies using other methods. When a DFS pattern is observed in IIF-ANA staining, it is more important to confirm the presence of other autoantibodies related to SARDs than to identify anti-DFS70 autoantibodies. Finally, more careful interpretation of IIF-ANA to specimens with a low IIF-ANA titer is needed.

• Authors: You La Jeon, So Young Kang, Woo-In Lee, Myeong Hee Kim
• Journal: Annals of clinical and laboratory science (Ann Clin Lab Sci) Vol. 49 Issue 4 Pg. 496-502 (Sep 2019) ISSN: 1550-8080 [Electronic] United States
• PMID: 31471339 (Publication Type: Journal Article)
• Copyright: © 2019 by the Association of Clinical Scientists, Inc.

Chemical References

• Adaptor Proteins, Signal Transducing
• Antibodies, Antinuclear
• Autoantibodies
• PSIP1 protein, human
• Transcription Factors

Topics

• Adaptor Proteins, Signal Transducing (immunology)
• Adolescent
• Adult
• Aged
• Aged, 80 and over
• Antibodies, Antinuclear (blood)
• Autoantibodies (blood)
• Autoimmune Diseases (blood, diagnosis, immunology)
• Child
• Child, Preschool
• Female
• Fluorescent Antibody Technique, Indirect
• Humans
• Male
• Mass Screening
• Middle Aged
• Transcription Factors (immunology)
• Young Adult