The group B Coxsackieviruses (CVB), belonging to the Enterovirus genus, can establish
persistent infections in human cells. These
persistent infections have been linked to
chronic diseases including type 1 diabetes. Still, the outcomes of persistent CVB
infections in human pancreas are largely unknown. We established persistent CVB
infections in a human pancreatic ductal-like cell line PANC-1 using two distinct CVB1 strains and profiled
infection-induced changes in cellular
protein expression and secretion using mass spectrometry-based proteomics.
Persistent infections, showing characteristics of carrier-state persistence, were associated with a broad spectrum of changes, including changes in mitochondrial network morphology and energy metabolism and in the regulated secretory pathway. Interestingly, the expression of
antiviral immune response
proteins, and also several other
proteins, differed clearly between the two
persistent infections. Our results provide extensive information about the
protein-level changes induced by persistent CVB
infection and the potential virus-associated variability in the outcomes of these
infections.