Specific changes in faecal microbiota are associated with familial Mediterranean fever.

Abstract

OBJECTIVES:

Familial Mediterranean fever (FMF) can be complicated by AA amyloidosis (AAA), though it remains unclear why only some patients develop amyloidosis. We examined the gut microbiota composition and inflammatory markers in patients with FMF complicated or not by AAA.

METHODS:

We analysed the gut microbiota of 34 patients with FMF without AAA, 7 patients with FMF with AAA, 19 patients with AAA of another origin, and 26 controls using 16S ribosomal RNA gene sequencing with the Illumina MiSeq platform. Associations between bacterial taxa and clinical phenotypes were evaluated using multivariate association with linear models statistical method. Blood levels of interleukin (IL)-1β, IL-6, tumour necrosis factor-α and adipokines were assessed by ELISA; indoleamine 2,3-dioxygenase (IDO) activity was determined by high-performance liquid chromatography.

RESULTS:

Compared with healthy subjects, specific changes in faecal microbiota were observed in FMF and AAA groups. Several operational taxonomic units (OTUs) were associated with FMF. Moreover, two OTUs were over-represented in FMF-related AAA compared with FMF without AAA. Additionally, higher adiponectin levels and IDO activity were observed in FMF-related AAA compared with FMF without AAA (p<0.05).

CONCLUSION:

The presence of specific changes in faecal microbiota in FMF and in FMF-related AAA suggests that intestinal microorganisms may play a role in the pathogenesis of these diseases. These findings may offer an opportunity to use techniques for gut microbiota manipulation.

• Authors: Samuel Deshayes, Soraya Fellahi, Jean-Philippe Bastard, Jean-Marie Launay, Jacques Callebert, Thibault Fraisse, David Buob, Jean-Jacques Boffa, Irina Giurgea, Charlotte Dupont, Sarah Jegou, Marjolène Straube, Alexandre Karras, Achille Aouba, Gilles Grateau, Harry Sokol, Sophie Georgin-Lavialle, AA Amyloidosis Study Group, AA amyloidosis Study Group
• Journal: Annals of the rheumatic diseases (Ann Rheum Dis) Vol. 78 Issue 10 Pg. 1398-1404 (10 2019) ISSN: 1468-2060 [Electronic] England
• PMID: 31377728 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © Author(s) (or their employer(s)) 2019. No commercial re-use. See rights and permissions. Published by BMJ.

Chemical References

• ADIPOQ protein, human
• Adiponectin
• Biomarkers
• Cytokines
• Inflammation Mediators

Topics

• Adiponectin (blood)
• Adult
• Aged
• Amyloidosis (microbiology)
• Biomarkers
• Cross-Sectional Studies
• Cytokines (blood)
• Enzyme-Linked Immunosorbent Assay
• Familial Mediterranean Fever (blood, microbiology)
• Feces (microbiology)
• Female
• Gastrointestinal Microbiome
• Humans
• Inflammation Mediators (blood)
• Linear Models
• Male
• Middle Aged
• Phenotype