Diagnostic performance of a single and duplicate Kato-Katz, Mini-FLOTAC, FECPAKG2 and qPCR for the detection and quantification of soil-transmitted helminths in three endemic countries.

Abstract

BACKGROUND:

Because the success of deworming programs targeting soil-transmitted helminths (STHs) is evaluated through the periodically assessment of prevalence and infection intensities, the use of the correct diagnostic method is of utmost importance. The STH community has recently published for each phase of a deworming program the minimal criteria that a potential diagnostic method needs to meet, the so-called target product profiles (TPPs).

METHODOLOGY:

We compared the diagnostic performance of a single Kato-Katz (reference method) with that of other microscopy-based methods (duplicate Kato-Katz, Mini-FLOTAC and FECPAKG2) and one DNA-based method (qPCR) for the detection and quantification of STH infections in three drug efficacy trials in Ethiopia, Lao PDR, and Tanzania. Furthermore, we evaluated a selection of minimal diagnostic criteria of the TPPs.

PRINCIPAL FINDINGS:

All diagnostic methods showed a clinical sensitivity of ≥90% for all STH infections of moderate-to-heavy intensities. For infections of very low intensity, only qPCR resulted in a sensitivity that was superior to a single Kato-Katz for all STHs. Compared to the reference method, both Mini-FLOTAC and FECPAKG2 resulted in significantly lower fecal egg counts for some STHs, leading to a substantial underestimation of the infection intensity. For qPCR, there was a positive significant correlation between the egg counts of a single Kato-Katz and the DNA concentration.

CONCLUSIONS/SIGNIFICANCE:

Our results indicate that the diagnostic performance of a single Kato-Katz is underestimated by the community and that diagnostic specific thresholds to classify intensity of infection are warranted for Mini-FLOTAC, FECPAKG2 and qPCR. When we strictly apply the TPPs, Kato-Katz is the only microscopy-based method that meets the minimal diagnostic criteria for application in the planning, monitoring and evaluation phase of an STH program. qPCR is the only method that could be considered in the phase that aims to seek confirmation for cessation of program.

TRIAL REGISTRATION:

ClinicalTrials.gov NCT03465488.

• Authors: Piet Cools, Johnny Vlaminck, Marco Albonico, Shaali Ame, Mio Ayana, Barrios Perez José Antonio, Giuseppe Cringoli, Daniel Dana, Jennifer Keiser, Maria P Maurelli, Catalina Maya, Leonardo F Matoso, Antonio Montresor, Zeleke Mekonnen, Greg Mirams, Rodrigo Corrêa-Oliveira, Simone A Pinto, Laura Rinaldi, Somphou Sayasone, Eurion Thomas, Jaco J Verweij, Jozef Vercruysse, Bruno Levecke
• Journal: PLoS neglected tropical diseases (PLoS Negl Trop Dis) Vol. 13 Issue 8 Pg. e0007446 (08 2019) ISSN: 1935-2735 [Electronic] United States
• PMID: 31369558 (Publication Type: Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Soil

Topics

• Adolescent
• Animals
• Brazil
• Child
• Diagnostic Tests, Routine (instrumentation, methods)
• Ethiopia (epidemiology)
• Feces (parasitology)
• Female
• Helminthiasis (diagnosis, epidemiology, parasitology, transmission)
• Helminths (genetics, isolation & purification)
• Humans
• Laos (epidemiology)
• Male
• Microscopy
• Molecular Diagnostic Techniques (instrumentation, methods)
• Parasite Egg Count (methods)
• Prevalence
• Real-Time Polymerase Chain Reaction (methods)
• Sensitivity and Specificity
• Soil (parasitology)
• Tanzania (epidemiology)
• World Health Organization