Fabricating antimicrobial peptide-immobilized starch sponges for hemorrhage control and antibacterial treatment.

Abstract

It is important to control immediate hemorrhage and prevent infection simultaneously in the wound management. However, most of hemostatic materials are associated with low efficiency of hemostasis, poor biocompatibility and lack of antimicrobial properties. A kind of starch-based macroporous sponges (KR-Sps) immobilized covalently with antimicrobial peptide KR12 using highly efficient thiol-ene photo click reaction were developed. The physical properties of these sponges could be fine-tuned by varying the ratio of modified starch/HS-PEG-SH and the polymer concentration. The in vitro and vivo results demonstrated that KR-Sps induced thrombosis, shortened clotting time and reduced the blood loss at bleeding site. Besides, KR12 immobilized sponge exhibited inherent antimicrobial properties against Gram (+) and (-) bacteria and methicillin-resistant Staphylococcus aureus (MRSA), which could maintain at least 5 days. Therefore, KR-Sps were believed to be an excellent candidate as hemostatic and antimicrobial product for the intraoperative wound management.

• Authors: Xiao Yang, Wen Liu, Guanghui Xi, Mingshan Wang, Bin Liang, Yifen Shi, Yakai Feng, Xiangkui Ren, Changcan Shi
• Journal: Carbohydrate polymers (Carbohydr Polym) Vol. 222 Pg. 115012 (Oct 15 2019) ISSN: 1879-1344 [Electronic] England
• PMID: 31320069 (Publication Type: Journal Article)
• Copyright: Copyright © 2019 Elsevier Ltd. All rights reserved.

Chemical References

• Anti-Bacterial Agents
• Antimicrobial Cationic Peptides
• Biocompatible Materials
• Hemostatics
• Immobilized Proteins
• Polyethylene Glycols
• Starch

Topics

• Animals
• Anti-Bacterial Agents (chemistry, pharmacology, toxicity)
• Antimicrobial Cationic Peptides (chemistry, pharmacology, toxicity)
• Biocompatible Materials (chemistry, pharmacology, toxicity)
• Escherichia coli (drug effects)
• Hemostatics (chemistry, pharmacology, toxicity)
• Immobilized Proteins (chemistry, pharmacology, toxicity)
• Male
• Methicillin-Resistant Staphylococcus aureus (drug effects)
• Mice
• Microbial Sensitivity Tests
• Polyethylene Glycols (chemistry, toxicity)
• Porosity
• Rats, Sprague-Dawley
• Solanum tuberosum (chemistry)
• Staphylococcus aureus (drug effects)
• Staphylococcus epidermidis (drug effects)
• Starch (chemistry, toxicity)
• Surgical Sponges