Polybrominated diphenyl ethers (
PBDEs) as potential neurotoxicants in environment may possess hazards to human health. Previous studies have reported that
PBDEs exposure could induce oxidative stress and disturb mitochondrial functions in mammalian cells. However, the toxicological mechanism remains to be clarified. In this work, the neurotoxic effect and underlying mechanism of
2,2',4,4'-tetrabromodiphenyl ether (BDE-47) was investigated by using human
neuroblastoma SK-N-SH cells as an effective model. A liquid chromatography-mass spectrometry (LC-MS)-based metabolomics approach combined with cell viability assay was applied to elucidate the metabolic perturbations and relevant toxicological pathways upon
BDE-47 exposure. Our results shown that the SK-N-SH cell viability decreased in a dose-dependent manner after exposure to
BDE-47 at 24 h within the concentration range of 5-250 μM, and an IC50 value of 88.8 μM was obtained. Based on the dose-response curve and cell morphological observation, the 5 and 10 μM
BDE-47 doses (equal to IC5 and IC10, respectively) were used for metabolomics study to capture the sensitive metabolic response following
BDE-47 exposure. After
BDE-47 treatment, nine metabolites were identified as potential
biomarkers, and the most disturbed metabolic pathways were mainly involved in
alanine,
aspartate and
glutamate metabolism,
glutathione metabolism,
tyrosine and
phenylalanine metabolism, and
pyrimidine metabolism, which imply that metabolic changes related to
neurotransmitters, oxidative stress, and
nucleotide-mediated signal transduction systems were the sensitive pathways mostly influenced. Our findings reported here may provide potential neurotoxic effect
biomarkers and prompt deep understanding of the molecular and metabolic mechanisms triggered by
BDE-47 exposure.