Freeze-dried Si-Ni-San powder can ameliorate high fat diet-induced non-alcoholic fatty liver disease.

Abstract

BACKGROUND:

Non-alcoholic fatty liver disease (NAFLD) is a common chronic liver disease worldwide. However, to date, there is no ideal therapy for this disease.

AIM:

To study the effects of Si-Ni-San freeze-dried powder on high fat diet-induced NAFLD in mice.

METHODS:

Twenty-four male C57BL/6 mice were randomized into three groups of eight. The control group (CON) was allowed ad libitum access to a normal chow diet. The high fat diet group (FAT) and Si-Ni-San group (SNS) were allowed ad libitum access to a high fat diet. The SNS group was intragastrically administered Si-Ni-San freeze-dried powder (5.0 g/kg) once daily, and the CON and FAT groups were intragastrically administered distilled water. After 12 wk, body weight, liver index, visceral fat index, serum alanine aminotransferase (ALT), portal lipopoly-saccharide (LPS), liver tumor necrosis factor (TNF)-α and liver triglycerides were measured. Intestinal microbiota were analyzed using a 16S r DNA sequencing technique.

RESULTS:

Compared with the FAT group, the SNS group exhibited decreased body weight, liver index, visceral fat index, serum ALT, portal LPS, liver TNF-α and liver triglycerides (P < 0.05). Intestinal microbiota analysis showed that the SNS group had different bacterial composition and function compared with the FAT group. In particular, Oscillospira genus was a bacterial biomarker of SNS group samples.

CONCLUSION:

The beneficial effects of Si-Ni-San freeze-dried powder on high fat diet-induced NAFLD in mice may be associated with its anti-inflammatory and changing intestinal microbiota effects.

• Authors: Feng Zhu, Yong-Min Li, Ting-Ting Feng, Yue Wu, Hai-Xia Zhang, Guo-Yin Jin, Jian-Ping Liu
• Journal: World journal of gastroenterology (World J Gastroenterol) Vol. 25 Issue 24 Pg. 3056-3068 (Jun 28 2019) ISSN: 2219-2840 [Electronic] United States
• PMID: 31293341 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents
• Drugs, Chinese Herbal
• Powders
• shigyaku-san

Topics

• Animals
• Anti-Inflammatory Agents (therapeutic use)
• Diet, High-Fat (adverse effects)
• Disease Models, Animal
• Drug Compounding (methods)
• Drug Evaluation, Preclinical
• Drugs, Chinese Herbal (therapeutic use)
• Freeze Drying
• Gastrointestinal Microbiome (drug effects, physiology)
• Humans
• Male
• Mice
• Non-alcoholic Fatty Liver Disease (drug therapy, etiology, microbiology)
• Powders
• Treatment Outcome