Peroxiredoxin V Reduces β-Lapachone-induced Apoptosis of Colon Cancer Cells.

Abstract

BACKGROUND/AIM:

Peroxiredoxin (Prx) V has been known as an antioxidant enzyme which scavenges intracellular reactive oxygen species (ROS). Also, Prx V has been shown to mediate cell apoptosis in various cancers. However, the mechanism of Prx V-induced apoptosis in colon cancer cells remains unknown. Thus, in this study we analyzed the effects of Prx V in β-lapachone-induced apoptosis in SW480 human colon cancer cells.

MATERIALS AND METHODS:

β-lapachone-induced apoptosis was analyzed by the MTT assay, western blotting, fluorescence microscopy, Annexin V staining and flow cytometry.

RESULTS:

Overexpression of Prx V, significantly decreased β-lapachone-induced cellular apoptosis and Prx V silencing increased β-lapachone-induced cellular apoptosis via modulating ROS scavenging activity compared to mock SW480 cells. In addition, to further explore the mechanism of Prx V regulated β-lapachone-induced SW480 cells apoptosis, the Wnt/β-catenin signaling was studied. The Wnt/ β-catenin signaling pathway was found to be induced by β-lapachone.

CONCLUSION:

Prx V regulates SW480 cell apoptosis via scavenging ROS cellular levels and mediating the Wnt/β-catenin signaling pathway, which was induced by β-lapachone.

• Authors: Yue Liu, Taeho Kwon, Ji-Su Kim, Nisansala Chandimali, Ying-Hua Jin, Yi-Xi Gong, Dan-Ping Xie, Ying-Hao Han, Mei-Hua Jin, Gui-Nan Shen, Dong Kee Jeong, Dong-Sun Lee, Yu-Dong Cui, Hu-Nan Sun
• Journal: Anticancer research (Anticancer Res) Vol. 39 Issue 7 Pg. 3677-3686 (Jul 2019) ISSN: 1791-7530 [Electronic] Greece
• PMID: 31262894 (Publication Type: Journal Article)
• Copyright: Copyright© 2019, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

Chemical References

• Naphthoquinones
• Reactive Oxygen Species
• beta-lapachone
• Peroxiredoxins

Topics

• Apoptosis
• Cell Line, Tumor
• Colon (metabolism)
• Colonic Neoplasms (metabolism)
• Humans
• Naphthoquinones
• Peroxiredoxins (physiology)
• Reactive Oxygen Species (metabolism)
• Wnt Signaling Pathway