Renal protective effects of aloin in a mouse model of sepsis.

Abstract

Aloin is the major anthraquinone glycoside obtained from the Aloe species and exhibits anti-inflammatory and anti-oxidative activities. However, the renal protective effects of aloin and underlying molecular mechanism remain unclear. This study was initiated to determine whether aloin could modulate renal functional damage in a mouse model of sepsis and to elucidate the underlying mechanisms. The potential of aloin treatment to reduce renal damage induced by cecal ligation and puncture (CLP) surgery in mice was measured by assessment of serum creatinine, blood urea nitrogen (BUN), lipid peroxidation, total glutathione, glutathione peroxidase activity, catalase activity, and superoxide dismutase activity. Post-treatment with aloin resulted in a significant reduction in the deleterious renal functions by CLP, such as elevated BUN, creatinine, and urine protein. Moreover, aloin inhibited nuclear factor-κB activation and reduced the induction of nitric oxide synthase and excessive production of nitric acid. Aloin treatment also reduced the plasma levels of interleukin-6 and tumor necrosis factor-α, reduced lethality due to CLP-induced sepsis, increased lipid peroxidation, and markedly enhanced the antioxidant defense system by restoring the levels of superoxide dismutase, glutathione peroxidase, and catalase in kidney tissues. Our study suggested that aloin protects mice against sepsis-triggered renal injury.

• Authors: Wonhwa Lee, Gil-Saeng Jeong, Moon-Chang Baek, Sae-Kwang Ku, Jong-Sup Bae
• Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (Food Chem Toxicol) Vol. 132 Pg. 110651 (Oct 2019) ISSN: 1873-6351 [Electronic] England
• PMID: 31254592 (Publication Type: Journal Article)
• Copyright: Copyright © 2019 Elsevier Ltd. All rights reserved.

Chemical References

• I-kappa B Proteins
• Interleukin-6
• NF-kappa B
• Protective Agents
• Tumor Necrosis Factor-alpha
• interleukin-6, mouse
• Peroxidase
• Nitric Oxide Synthase Type II
• Nos2 protein, mouse
• Emodin
• alloin

Topics

• Animals
• Cell Line
• Emodin (analogs & derivatives, pharmacology)
• I-kappa B Proteins (metabolism)
• Interleukin-6 (metabolism)
• Kidney (injuries, pathology)
• Kidney Diseases (prevention & control)
• Lipid Peroxidation (drug effects)
• Male
• Mice, Inbred C57BL
• NF-kappa B (metabolism)
• Nitric Oxide Synthase Type II (metabolism)
• Oxidative Stress (drug effects)
• Peroxidase (metabolism)
• Protective Agents (pharmacology)
• Sepsis (prevention & control)
• Tumor Necrosis Factor-alpha (metabolism)