Oxidative stress biomarkers in the serum and plasma of patients with non-alcoholic fatty liver disease (NAFLD). Can plasma AGE be a marker of NAFLD? Oxidative stress biomarkers in NAFLD patients.

Abstract

Still little is known about the redox abnormalities in patients with non-alcoholic fatty liver disease (NAFLD). The purpose of the study was to find the relationship between enzymatic and non-enzymatic antioxidants, redox homeostasis and oxidative damage in 67-patients with NAFLD. The study population was divided into patients with non-alcoholic fatty liver (early NAFLD, n = 29) and patients with non-alcoholic steatohepatitis (advanced NAFLD, n = 38). Redox biomarkers: enzymatic antioxidants (Cu - Zn-superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR)); non-enzymatic antioxidants and redox status (reduced glutathione (GSH), total antioxidant capacity (TAC)); and oxidative damage products (total oxidant status (TOS), advanced glycation end products (AGE), malondialdehyde (MDA), and DNA/RNA oxidative damage) were determined in the serum/plasma samples. The activity of SOD, GPx, GR and levels of GSH, TOS, AGE, MDA, and DNA/RNA oxidative damage were significantly elevated in early NAFLD and advanced NAFLD group compared to controls (p < .001). There was a positive correlation between AGE, TAC and ALT activity (R = 0.34, p = .04; R = 0.36, p = .03, respectively) in advanced NAFLD group. Interestingly, ROC analysis for AGE showed good discriminatory ratio for patients with minimal steatosis (BARD score 0-1) vs. moderate steatosis (BARD score 2-4), AUC = 0.76. Plasma AGE can be a potential non-invasive biomarker differentiating NAFLD patients.

• Authors: Magdalena Świderska, Mateusz Maciejczyk, Anna Zalewska, Joanna Pogorzelska, Robert Flisiak, Adrian Chabowski
• Journal: Free radical research (Free Radic Res) Vol. 53 Issue 8 Pg. 841-850 (Aug 2019) ISSN: 1029-2470 [Electronic] England
• PMID: 31234658 (Publication Type: Journal Article)

Chemical References

• Antioxidants
• Biomarkers
• Glycation End Products, Advanced
• Malondialdehyde
• Catalase
• Glutathione Peroxidase
• Superoxide Dismutase
• Glutathione Reductase
• Glutathione

Topics

• Adult
• Antioxidants (analysis)
• Biomarkers (blood)
• Catalase (blood)
• Female
• Glutathione (blood)
• Glutathione Peroxidase (blood)
• Glutathione Reductase (blood)
• Glycation End Products, Advanced (blood)
• Humans
• Male
• Malondialdehyde (analysis)
• Middle Aged
• Non-alcoholic Fatty Liver Disease (blood, metabolism)
• Oxidative Stress
• Superoxide Dismutase (blood)