Hydrogen sulfide impacts on inflammation-induced adipocyte dysfunction.

Abstract

A dual role of hydrogen sulfide (H2S) in inflammation is well-reported and recent studies demonstrated adipogenic effects of H2S in 3T3-L1 cells. Here, we aimed to investigate the effects of H2S on adipocyte differentiation and inflammation. H2S concentration in 3T3-L1 culture media was increased during adipocyte differentiation in parallel to adipogenic and Cth gene expression, and its inhibition using DL-Propargyl Glycine (PPG) impaired 3T3-L1 differentiation. GYY4137 and Na2S administration only in the first or in the last stage of adipocyte differentiation resulted in a significant increased expression of adipogenic genes. However, when GYY4137 or Na2S were administrated during all process no significant effects on adipogenic gene expression were found, suggesting that excessive H2S administration might exert negative effects on adipogenesis. In fact, continuous addition of Na2S, which resulted in Na2S excess, inhibited adipogenesis, whereas time-expired Na2S had no effect. In inflammatory conditions, GYY4137, but not Na2S, administration attenuated the negative effects of inflammation on adipogenesis and insulin signaling-related gene expression during adipocyte differentiation. In inflamed adipocytes, Na2S administration enhanced the negative effects of inflammatory process. Altogether these data showed that slow-releasing H2S improved adipocyte differentiation in inflammatory conditions, and that H2S proadipogenic effects depend on dose, donor and exposure time.

• Authors: Ferran Comas, Jèssica Latorre, Olaf Cussó, Francisco Ortega, Aina Lluch, Mònica Sabater, Anna Castells-Nobau, Wifredo Ricart, Xavier Ribas, Miquel Costas, José Manuel Fernández-Real, José María Moreno-Navarrete
• Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (Food Chem Toxicol) Vol. 131 Pg. 110543 (Sep 2019) ISSN: 1873-6351 [Electronic] England
• PMID: 31154084 (Publication Type: Journal Article)
• Copyright: Copyright © 2019 Elsevier Ltd. All rights reserved.

Chemical References

• Alkynes
• GYY 4137
• Morpholines
• Organothiophosphorus Compounds
• Sulfides
• propargylglycine
• Glycine
• sodium sulfide
• Hydrogen Sulfide

Topics

• 3T3-L1 Cells
• Adipocytes (drug effects)
• Adipogenesis (drug effects, physiology)
• Alkynes (pharmacology)
• Animals
• Cell Differentiation (drug effects)
• Gene Expression (drug effects)
• Glycine (analogs & derivatives, pharmacology)
• Hydrogen Sulfide (metabolism)
• Inflammation (physiopathology)
• Mice
• Morpholines (pharmacology)
• Organothiophosphorus Compounds (pharmacology)
• Sulfides (pharmacology)