Novel Diamide-Based Benzenesulfonamides as Selective Carbonic Anhydrase IX Inhibitors Endowed with Antitumor Activity: Synthesis, Biological Evaluation and In Silico Insights.

Abstract

In this work, we present the synthesis and biological evaluation of novel series of diamide-based benzenesulfonamides 5a-h as inhibitors of the metalloenzyme carbonic anhydrase (CA, EC 4.2.1.1) isoforms hCA I, II, IX and XII. The target tumor-associated isoforms hCA IX and XII were undeniably the most affected ones (KIs: 8.3-123.3 and 9.8-134.5 nM, respectively). Notably, diamides 5a and 5h stood out as a single-digit nanomolar hCA IX inhibitors (KIs = 8.8 and 8.3 nM). The SAR outcomes highlighted that bioisosteric replacement of the benzylidene moiety, compounds 5a-g, with the hetero 2-furylidene moiety, compound 5h, achieved the best IX/I and IX/II selectivity herein reported with SIs of 985 and 13.8, respectively. Molecular docking simulations of the prepared diamides within CA IX active site revealed the ability of 5h to establish an additional H-bond between the heterocyclic oxygen and HE/Gln67. Moreover, benzenesulfonamides 5a, 5b and 5h were evaluated for their antitumor activity against renal cancer UO-31 cell line. Compound 5h was the most potent derivative with about 1.5-fold more enhanced activity (IC50 = 4.89 ± 0.22 μM) than the reference drug Staurosporine (IC50 = 7.25 ± 0.43 μM). Moreover, 5a and 5h were able to induce apoptosis in UO-31 cells as evidenced by the significant increase in the percent of annexinV-FITC positive apoptotic cells by 22.5- and 26.5-folds, respectively.

• Authors: Mohamed A Abdelrahman, Wagdy M Eldehna, Alessio Nocentini, Silvia Bua, Sara T Al-Rashood, Ghada S Hassan, Alessandro Bonardi, Abdulrahman A Almehizia, Hamad M Alkahtani, Amal Alharbi, Paola Gratteri, Claudiu T Supuran
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 20 Issue 10 (May 20 2019) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 31137489 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• Benzene Derivatives
• Carbonic Anhydrase Inhibitors
• Sulfonamides
• Carbonic Anhydrase IX

Topics

• Antineoplastic Agents (chemical synthesis, pharmacology)
• Benzene Derivatives (chemistry)
• Binding Sites
• Carbonic Anhydrase IX (antagonists & inhibitors, chemistry, metabolism)
• Carbonic Anhydrase Inhibitors (chemical synthesis, pharmacology)
• Cell Line, Tumor
• Humans
• Molecular Docking Simulation
• Protein Binding
• Sulfonamides (chemistry)