Potential Forensic Application of Receptor for Advanced Glycation End Products (RAGE) as a Novel Biomarker for Estimating Postmortem Interval.

Abstract

Studies which estimate the postmortem interval (PMI) based on diatoms or bacterial communities have been well-conducted. However, predicting the PMI based on microorganisms has limited accuracy. The objective of this study was to discover specific biomarkers for estimating the PMI for drowning. The in vivo experimental model consisted of rats classified into nine groups (seven drowned groups from Day 1 to Day 7 and two control groups). Their lung tissues were analyzed for the receptor for advanced glycation end products (RAGE) mRNA expression determined by qRT-PCR. RAGE protein levels were determined by Western blotting and immunohistochemical (IHC) staining. RAGE mRNA and protein levels gradually degraded over time. Immunostaining analysis revealed that RAGE in the Day 1 sample was significantly higher than that in the Day 6 or Day 7 samples. These results indicate that RAGE in drowned lungs could be a potential biomarker for the determination of the PMI after drowning.

• Authors: Hye-Won Cho, Yong-Bin Eom
• Journal: Journal of forensic sciences (J Forensic Sci) Vol. 64 Issue 6 Pg. 1878-1883 (Nov 2019) ISSN: 1556-4029 [Electronic] United States
• PMID: 30990889 (Publication Type: Journal Article)
• Copyright: © 2019 American Academy of Forensic Sciences.

Chemical References

• Biomarkers
• RNA, Messenger
• Receptor for Advanced Glycation End Products

Topics

• Animals
• Biomarkers (metabolism)
• Blotting, Western
• Drowning (diagnosis)
• Forensic Pathology
• Immunohistochemistry
• Lung (metabolism)
• Models, Animal
• Postmortem Changes
• RNA, Messenger (metabolism)
• Rats, Sprague-Dawley
• Receptor for Advanced Glycation End Products (genetics, metabolism)
• Reverse Transcriptase Polymerase Chain Reaction