5-Aminolevulinic
acid (5-ALA), a commonly used
photosensitizer in photodynamic detection (PDD) and
therapy (
PDT), is converted in situ to the established
photosensitizer protoporphyrin IX (
PpIX) via the
heme biosynthetic pathway. To extend 5-ALA-PDT application, we evaluated the
PpIX fluorescence induced by exogenous 5-ALA in various veterinary
tumors and treated canine and feline
tumors. 5-ALA-PDD sensitivity and specificity in the whole sample group for dogs and cats combined were 89.5 and 50%, respectively. Notably, some small
tumors disappeared upon 5-ALA-PDT. Although single
PDT application was not curative, repeated
PDT+/-
chemotherapy achieved long-term
tumor control. We analyzed the relationship between intracellular
PpIX concentration and 5-ALA-PDT in vitro cytotoxicity using various primary
tumor cells and determined the correlation between intracellular
PpIX concentration and 5-ALA transporter and metabolic
enzyme mRNA expression levels. 5-ALA-PDT cytotoxicity in vitro correlated with intracellular
PpIX concentration in
carcinomas.
Ferrochelatase mRNA expression levels strongly negatively correlated with
PpIX accumulation, representing the first report of a correlation between
mRNA expression related to
PpIX accumulation and
PpIX concentration in canine
tumor cells. Our findings suggested that the results of 5-ALA-PDD might be predictive for 5-ALA-PDT
therapeutic effects for
carcinomas, with 5-ALA-PDT plus
chemotherapy potentially increasing the probability of
tumor control in veterinary medicine.