Using recursive partitioning approach to select tumor-associated antigens in immunodiagnosis of gastric adenocarcinoma.

Abstract

The present study aimed to select anti-tumor-associated antigen (TAA) autoantibodies as biomarkers in the immunodiagnosis of gastric adenocarcinoma (GAC) by the recursive partitioning approach (RPA) and further construct and evaluate a predictive model. A case-control study was designed including 407 GAC patients as the case group and 407 normal controls. In addition, 67 serial serum samples from 25 GAC patients were collected at different time points before and after gastrectomy treatment. Autoantibodies against 14 TAA were measured in sera from all subjects by enzyme immunoassay. Finally, RPA resulted in the selection of nine-panel TAA (c-Myc, p16, HSPD1, PTEN, p53, NPM1, ENO1, p62, HCC1.4) from all detected TAA in the case-control study; the classification tree based on this nine-TAA panel had area under curve (AUC) of 0.857, sensitivity of 71.5% and specificity of 71.3%; The optimal panel also can identify GAC patients at an early stage from normal individuals, with AUC of 0.737, sensitivity of 64.9% and specificity of 70.5%. However, frequencies of the nine autoantibodies showed no correlation with GAC stage, tumor size, lymphatic metastasis or differentiation. GAC patients positive for more than two autoantibodies in the nine-TAA panel had a worse prognosis than that of the GAC patients positive for no or one antibody. Titers of 10 autoantibodies in serial serum samples were significantly higher in GAC patients after surgical resection than before. In conclusion, this study showed that the panel of nine multiple TAAs could enhance the detection of anti-TAA antibodies in GAC, and may be potential prognostic biomarkers in GAC.

• Authors: Jiejie Qin, Shuaibing Wang, Jianxiang Shi, Yan Ma, Keyan Wang, Hua Ye, Xiaojun Zhang, Peng Wang, Xiao Wang, Chunhua Song, Liping Dai, Kaijuan Wang, Binghua Jiang, Jianying Zhang
• Journal: Cancer science (Cancer Sci) Vol. 110 Issue 6 Pg. 1829-1841 (Jun 2019) ISSN: 1349-7006 [Electronic] England
• PMID: 30950146 (Publication Type: Journal Article)
• Copyright: © 2019 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

Chemical References

• Antigens, Neoplasm
• Autoantibodies
• Biomarkers, Tumor
• NPM1 protein, human
• Nucleophosmin

Topics

• Adenocarcinoma (diagnosis, immunology)
• Adult
• Aged
• Aged, 80 and over
• Antigens, Neoplasm (blood, immunology)
• Autoantibodies (blood, immunology)
• Biomarkers, Tumor (blood, immunology)
• Case-Control Studies
• Female
• Humans
• Immunologic Tests (methods)
• Male
• Middle Aged
• Multivariate Analysis
• Nucleophosmin
• ROC Curve
• Stomach Neoplasms (diagnosis, immunology)
• Young Adult