The protective effect of oleanolic acid on NMDA-induced MLE-12 cells apoptosis and lung injury in mice by activating SIRT1 and reducing NF-κB acetylation.

Abstract

Overactivation of the N-methyl-d-aspartate (NMDA) receptor promotes oxidative stress, aggravates the inflammatory response and induces excitotoxic lung injury. NMDA is a synthetic agonist that selectively activates the NMDA receptor. Oleanolic acid (OA) is a natural anti-inflammatory and antioxidant compound. This study investigated the effect and possible mechanism of OA on NMDA-induced acute lung injury (ALI) in mice. OA pretreatment alleviated NMDA-induced histological lung changes and ameliorated pulmonary oedema and pulmonary permeability. At the same time, OA inhibited inflammatory cell infiltration and decreased the levels of tumour necrosis factor (TNF)-α, interleukin (IL)-6 and IL-1β in the lung and bronchoalveolar lavage fluid (BALF). OA markedly decreased malondialdehyde (MDA) production and increased the superoxide dismutase (SOD) and glutathione (GSH) contents of the lung in vivo. Meanwhile, we first found that NMDA increased LDH activity and decreased cell viability, and induced oxidative stress and apoptosis in mouse lung epithelial (MLE)-12 cells. By employing SRT1720 and sirtinol, the activator and inhibitor of sirtuin 1 (SIRT1), we found that SRT1720 partially eliminated the increase in ROS，and sirtinol further promoted the increase in ROS caused by NMDA. OA increased MLE-12 cells viability and attenuated oxidative stress after NMDA challenge in vitro. OA suppressed NMDA-induced MLE-12 cells apoptosis, while sirtinol inhibited the effect of OA. In addition, OA significantly upregulated the levels of SIRT1, nuclear-related factor 2(Nrf2) and Bcl-2 protein and downregulated the levels of acetylated nuclear factor-kappa B (NF-κB), NLRP3 and Bax protein. In conclusion, OA attenuated NMDA-induced excitotoxic lung injury, potentially through its anti-inflammatory, antioxidative stress and anti-apoptotic effects. The mechanism may be related to activating SIRT1 and reducing NF-κB acetylation.

• Authors: Xiang-Ping Peng, Xiao-Hong Li, Yang Li, Xiao-Ting Huang, Zi-Qiang Luo
• Journal: International immunopharmacology (Int Immunopharmacol) Vol. 70 Pg. 520-529 (May 2019) ISSN: 1878-1705 [Electronic] Netherlands
• PMID: 30901738 (Publication Type: Journal Article)
• Copyright: Copyright © 2019 Elsevier B.V. All rights reserved.

Chemical References

• Anti-Inflammatory Agents
• NF-kappa B
• N-Methylaspartate
• Oleanolic Acid
• Sirtuin 1

Topics

• Acetylation
• Acute Lung Injury (chemically induced, drug therapy)
• Animals
• Anti-Inflammatory Agents (therapeutic use)
• Apoptosis (drug effects)
• Cell Line
• Disease Models, Animal
• Gene Expression Regulation
• Humans
• Male
• Mice
• Mice, Inbred BALB C
• N-Methylaspartate (metabolism)
• NF-kappa B (metabolism)
• Oleanolic Acid (therapeutic use)
• Oxidative Stress (drug effects)
• Respiratory Mucosa (physiology)
• Sirtuin 1 (genetics, metabolism)