HIV-1 infection modulates IL-24 expression which contributes to cell apoptosis in vitro.

Abstract

Although interleukin-24 (IL-24) has been extensively explored in the immunopathologies of autoimmune diseases, neoplasms, and infections, its role in HIV-1 infection has not been thoroughly elucidated to date. Therefore, the objective of this study was to evaluate the gene and protein expressions of IL-24 at the initial moments of HIV infection in PBMCs. Due to the pro-apoptotic role of IL-24, we evaluated the protein expression of caspase-3, as well as Annexin V/Propidium Iodide flow cytometry and phosphorylation of ERK, which may induce an apoptotic signal block when phosphorylated. The results of this study demonstrated that HIV-1 infection had an impact on the gene and protein expressions of IL-24 and ERK. Annexin V/Propidium Iodide assay demonstrated decrease in the mechanisms of apoptosis in infected cells after incubation of IL-24 neutralizing antibody. Studies on how HIV-1 regulates IL-24 expression may play a role in characterizing viral persistence mechanisms and designing antiretroviral strategies.

• Authors: Scheilla Teixeira Strumillo, Marli Ferreira Curcio, Fábio Ferreira de Carvalho Jr, MariaCecília Araripe Sucupira, Ricardo Sobhie Diaz, Hugo Pequeno Monteiro, Luiz Mário Ramos Janini
• Journal: Cell biology international (Cell Biol Int) Vol. 43 Issue 5 Pg. 574-579 (May 2019) ISSN: 1095-8355 [Electronic] England
• PMID: 30761646 (Publication Type: Journal Article)
• Copyright: © 2019 International Federation for Cell Biology.

Chemical References

• Annexin A5
• Interleukins
• diannexin, human
• interleukin-24
• MAPK3 protein, human
• Mitogen-Activated Protein Kinase 3
• CASP3 protein, human
• Caspase 3

Topics

• Annexin A5 (immunology)
• Apoptosis (physiology)
• Blood Cells (immunology)
• Caspase 3 (immunology)
• HIV Infections (immunology)
• HIV-1 (immunology)
• Humans
• Interleukins (immunology)
• Mitogen-Activated Protein Kinase 3 (immunology)
• Primary Cell Culture