Essential roles of C-type lectin Mincle in induction of neuropathic pain in mice.

Abstract

Increasing evidence indicates that pattern recognition receptors (PRRs) are involved in neuropathic pain after peripheral nerve injury (PNI). While a significant number of studies support an association between neuropathic pain and the innate immune response mediated through Toll-like receptors, a family of PRRs, the roles of other types of PRRs are largely unknown. In this study, we have focused on the macrophage-inducible C-type lectin (Mincle), a PRR allocated to the C-type lectin receptor family. Here, we show that Mincle is involved in neuropathic pain after PNI. Mincle-deficient mice showed impaired PNI-induced mechanical allodynia. After PNI, expression of Mincle mRNA was rapidly increased in the injured spinal nerve. Most Mincle-expressing cells were identified as infiltrating leucocytes, although the migration of leucocytes was also observed in Mincle-deficient mice. Furthermore, Mincle-deficiency affected the induction of genes, which are reported to contribute to neuropathic pain after PNI in the dorsal root ganglia and spinal dorsal horn. These results suggest that Mincle is involved in triggering sequential processes that lead to the pathogenesis of neuropathic pain.

• Authors: Asako Ishikawa, Yasunobu Miyake, Kimiko Kobayashi, Yuzo Murata, Sayaka Iizasa, Ei'ichi Iizasa, Sho Yamasaki, Naomi Hirakawa, Hiromitsu Hara, Hiroki Yoshida, Toshiharu Yasaka
• Journal: Scientific reports (Sci Rep) Vol. 9 Issue 1 Pg. 872 (01 29 2019) ISSN: 2045-2322 [Electronic] England
• PMID: 30696945 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Clecsf8 protein, mouse
• Lectins, C-Type
• Membrane Proteins
• Receptors, Pattern Recognition
• Toll-Like Receptors

Topics

• Animals
• Disease Models, Animal
• Ganglia, Spinal (metabolism)
• Hyperalgesia (metabolism)
• Immunity, Innate
• Lectins, C-Type (metabolism, physiology)
• Macrophages (metabolism)
• Male
• Membrane Proteins (metabolism, physiology)
• Mice
• Mice, Inbred C57BL
• Neuralgia (metabolism, physiopathology)
• Peripheral Nerve Injuries (metabolism, physiopathology)
• Receptors, Pattern Recognition (metabolism)
• Spinal Cord Dorsal Horn (metabolism)
• Spinal Nerves (pathology)
• Toll-Like Receptors (metabolism)