Abstract | BACKGROUND: HYPOTHESIS: AIM: We assessed the effects of selected adaptogenic herbal extracts on FEC (fixed combination 5-fluorouracil, epirubicin and cyclophosphamide) induced changes in transcriptome-wide RNA microarray profiles of neuroglia cells. The aim of the study was to predict potential effects of andrographolide, Andrographis herb, Eleutherococcus root genuine extracts, their fixed combination (AE) and the combination of Rhodiola roots, Schisandra berries and Eleutherococcus roots (RSE) on cellular and physiological, mostly cognitive functions. METHODS: Gene expression profiling was performed by transcriptome-wide mRNA microarray in the human T98G neuroglia cells after treatment with adaptogens. Interactive pathways downstream analysis was performed with data sets of significantly up- or down-regulated genes and predicted effects on cellular functions and diseases were identified by Ingenuity IPA database software. RESULTS: FEC deregulated 67 genes involved in decrease of neuronal development, 37 genes involved in development of the sensory system, 12 genes in extension of axons, and 3 genes in migration of neurons. Co-incubation with Andrographis paniculata (AP) suppressed FEC-induced deregulation of a large number of genes involved in predicted activation of neuronal death and inhibition of neurogenesis, and 16 genes related to inhibition of several functions in the nervous system. Co-incubation with AE suppressed FEC-induced deregulation of a number of genes involved in predicted inhibition of axon extension, migration of T98G neuroglia cells, conduction of nerves and other genes related to regulations of some other functions in the nervous system. CONCLUSION:
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Authors | Ean-Jeong Seo, Sabine M Klauck, Thomas Efferth, Alexander Panossian |
Journal | Phytomedicine : international journal of phytotherapy and phytopharmacology
(Phytomedicine)
Vol. 55
Pg. 80-91
(Mar 01 2019)
ISSN: 1618-095X [Electronic] Germany |
PMID | 30668446
(Publication Type: Journal Article)
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Copyright | Copyright © 2018 Elsevier GmbH. All rights reserved. |
Chemical References |
- Diterpenes
- Plant Extracts
- Epirubicin
- andrographolide
- Cyclophosphamide
- Fluorouracil
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Topics |
- Andrographis
(chemistry)
- Antineoplastic Combined Chemotherapy Protocols
(adverse effects)
- Cell Line
- Cognitive Dysfunction
(chemically induced, drug therapy, genetics)
- Cyclophosphamide
(adverse effects)
- Diterpenes
(pharmacology)
- Epirubicin
(adverse effects)
- Fluorouracil
(adverse effects)
- Fruit
(chemistry)
- Gene Expression Profiling
(methods)
- Gene Expression Regulation
(drug effects)
- Humans
- Neuroglia
(drug effects, physiology)
- Neurotoxicity Syndromes
(genetics)
- Oligonucleotide Array Sequence Analysis
(methods)
- Plant Extracts
(pharmacology)
- Rhodiola
(chemistry)
- Schisandra
(chemistry)
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