IL-6 in Osteoarthritis: Effects of Pine Stilbenoids.

Abstract

Interleukin-6 (IL-6) is involved in the pathogenesis of various inflammatory diseases, like rheumatoid arthritis (RA). In the present study, we investigated the role of IL-6 in osteoarthritis (OA) patients and the effects of the stilbenoids monomethyl pinosylvin and pinosylvin on the expression of the cartilage matrix components aggrecan and collagen II and the inflammatory cytokine IL-6 in human OA chondrocytes. Synovial fluid and plasma samples were obtained from 100 patients with severe OA [BMI 29.7 (8.3) kg/m², age 72 (14) years, median (IQR); 62/38 females/males] undergoing total knee replacement surgery. IL-6 and matrix metalloproteinase (MMP) concentrations in synovial fluid and plasma were measured by immunoassay. The effects of pinosylvin on the expression of IL-6, aggrecan, and collagen II were studied in primary cultures of human OA chondrocytes. IL-6 levels in synovial fluid from OA patients [119.8 (193.5) pg/mL, median (IQR)] were significantly increased as compared to the plasma levels [3.1 (2.7) pg/mL, median (IQR)] and IL-6 levels in synovial fluid were associated with MMPs and radiographic disease severity. Natural stilbenoids monomethyl pinosylvin and pinosylvin increased aggrecan expression and suppressed IL-6 production in OA chondrocytes. The results propose that IL-6 is produced within OA joints and has an important role in the pathogenesis of OA. Stilbenoid compounds monomethyl pinosylvin and pinosylvin appeared to have disease-modifying potential in OA chondrocytes.

• Authors: Mirka Laavola, Tiina Leppänen, Mari Hämäläinen, Katriina Vuolteenaho, Teemu Moilanen, Riina Nieminen, Eeva Moilanen
• Journal: Molecules (Basel, Switzerland) (Molecules) Vol. 24 Issue 1 (Dec 29 2018) ISSN: 1420-3049 [Electronic] Switzerland
• PMID: 30597965 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents
• Biomarkers
• Cytokines
• Inflammation Mediators
• Interleukin-6
• NF-kappa B
• Plant Extracts
• Stilbenes
• Collagen
• Matrix Metalloproteinases
• Resveratrol

Topics

• Aged
• Aged, 80 and over
• Anti-Inflammatory Agents (pharmacology)
• Biomarkers
• Cartilage, Articular (metabolism, pathology)
• Cell Line
• Cells, Cultured
• Chondrocytes (drug effects, metabolism)
• Collagen (metabolism)
• Cytokines (metabolism)
• Female
• Gene Expression
• Humans
• Inflammation Mediators (metabolism)
• Interleukin-6 (blood, metabolism)
• Male
• Matrix Metalloproteinases (metabolism)
• Middle Aged
• NF-kappa B (metabolism)
• Osteoarthritis (blood, diagnostic imaging, metabolism, pathology)
• Pinus (chemistry)
• Plant Extracts (chemistry, pharmacology)
• Resveratrol (pharmacology)
• Severity of Illness Index
• Stilbenes (chemistry, pharmacology)
• Synovial Fluid (metabolism)